The DEP1 Mutation Improves Stem Lodging Resistance and Biomass Saccharification by Affecting Cell Wall Biosynthesis in Rice

Ye Wang; Meihan Wang; Xia Yan; Kaixuan Chen; Fuhao Tian; Xiao Yang; Liyu Cao; Nan Ruan; Zhengjun Dang; Xuelin Yin; Yuwei Huang; Fengcheng Li; Quan Xu

doi:10.1186/s12284-024-00712-0

The DEP1 Mutation Improves Stem Lodging Resistance and Biomass Saccharification by Affecting Cell Wall Biosynthesis in Rice

Rice (N Y). 2024 May 15;17(1):35. doi: 10.1186/s12284-024-00712-0.

Authors

Ye Wang^#¹, Meihan Wang^#¹, Xia Yan¹, Kaixuan Chen¹, Fuhao Tian¹, Xiao Yang¹, Liyu Cao¹, Nan Ruan¹, Zhengjun Dang¹, Xuelin Yin¹, Yuwei Huang¹, Fengcheng Li², Quan Xu³

Affiliations

¹ Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang Agricultural University, Shenyang, China.
² Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang Agricultural University, Shenyang, China. lifc_1983@163.com.
³ Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang Agricultural University, Shenyang, China. kobexu34@syau.edu.cn.

^# Contributed equally.

Abstract

Background: Plant cell walls have evolved precise plasticity in response to environmental stimuli. The plant heterotrimeric G protein complexes could sense and transmit extracellular signals to intracellular signaling systems, and activate a series of downstream responses. dep1 (Dense and Erect Panicles 1), the gain-of-function mutation of DEP1 encoding a G protein γ subunit, confers rice multiple improved agronomic traits. However, the effects of DEP1 on cell wall biosynthesis and wall-related agronomic traits remain largely unknown.

Results: In this study, we showed that the DEP1 mutation affects cell wall biosynthesis, leading to improved lodging resistance and biomass saccharification. The DEP1 is ubiquitously expressed with a relatively higher expression level in tissues rich in cell walls. The CRISPR/Cas9 editing mutants of DEP1 (dep1-cs) displayed a significant enhancement in stem mechanical properties relative to the wild-type, leading to a substantial improvement in lodging resistance. Cell wall analyses showed that the DEP1 mutation increased the contents of cellulose, hemicelluloses, and pectin, and reduced lignin content and cellulose crystallinity (CrI). Additionally, the dep1-cs seedlings exhibited higher sensitivity to cellulose biosynthesis inhibitors, 2,6-Dichlorobenzonitrile (DCB) and isoxaben, compared with the wild-type, confirming the role of DEP1 in cellulose deposition. Moreover, the DEP1 mutation-mediated alterations of cell walls lead to increased enzymatic saccharification of biomass after the alkali pretreatment. Furthermore, the comparative transcriptome analysis revealed that the DEP1 mutation substantially altered expression of genes involved in carbohydrate metabolism, and cell wall biosynthesis.

Conclusions: Our findings revealed the roles of DEP1 in cell wall biosynthesis, lodging resistance, and biomass saccharification in rice and suggested genetic modification of DEP1 as a potential strategy to develop energy rice varieties with high lodging resistance.

Keywords: DEP1; Biomass Saccharification; Cell wall; Lodging Resistance; Rice.