The impact of Sangju Qingjie Decoction on the pulmonary microbiota in the prevention and treatment of chronic obstructive pulmonary disease

Front Cell Infect Microbiol. 2024 Apr 30:14:1379831. doi: 10.3389/fcimb.2024.1379831. eCollection 2024.

Abstract

Objective: Exploring the effect of SJQJD on the pulmonary microbiota of chronic obstructive pulmonary disease (COPD) rats through 16S ribosomal RNA (rRNA) sequencing.

Methods: A COPD rat model was constructed through smoking and lipopolysaccharide (LPS) stimulation, and the efficacy of SJQJD was evaluated by hematoxylin and eosin (H&E) staining and Enzyme-Linked Immunosorbnent Assay (ELISA). The alveolar lavage fluid of rats was subjected to 16S rRNA sequencing. The diversity of lung microbiota composition and community structure was analyzed and differential microbiota were screened. Additionally, machine learning algorithms were used for screening biomarkers of each group of the microbiota.

Results: SJQJD could improve lung structure and inflammatory response in COPD rats. 16s rRNA sequencing analysis showed that SJQJD could significantly improve the abundance and diversity of bacterial communities in COPD rats. Through differential analysis and machine learning methods, potential microbial biomarkers were identified as Mycoplasmataceae, Bacillaceae, and Lachnospiraceae.

Conclusion: SJQJD could improve tissue morphology and local inflammatory response in COPD rats, and its effect may be related to improve pulmonary microbiota.

Keywords: COPD; SJQJD; biomarker; machine learning; pulmonary microbiota.

MeSH terms

  • Animals
  • Bacteria / classification
  • Bacteria / drug effects
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Bronchoalveolar Lavage Fluid / microbiology
  • Disease Models, Animal*
  • Drugs, Chinese Herbal* / pharmacology
  • Drugs, Chinese Herbal* / therapeutic use
  • Lung* / microbiology
  • Lung* / pathology
  • Male
  • Microbiota* / drug effects
  • Pulmonary Disease, Chronic Obstructive* / drug therapy
  • Pulmonary Disease, Chronic Obstructive* / microbiology
  • RNA, Ribosomal, 16S* / genetics
  • Rats
  • Rats, Sprague-Dawley

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by projects SF-YB20231022 and SF-YB20211018 from the Science and Technology Bureau of Xiangtan City, Scientific Research Program of Health Commission of Hunan Province, (No. B2017166).