Electrochemical aptasensors based on porous carbon derived from graphene oxide/ZIF-8 composites for the detection of Erwinia cypripedii

Jiayue Sun; Lei Ji; Yan Li; Xiyue Cao; Xiuling Shao; Jianfei Xia; Zonghua Wang

doi:10.1016/j.talanta.2024.126250

Electrochemical aptasensors based on porous carbon derived from graphene oxide/ZIF-8 composites for the detection of Erwinia cypripedii

Talanta. 2024 May 11:276:126250. doi: 10.1016/j.talanta.2024.126250. Online ahead of print.

Authors

Jiayue Sun¹, Lei Ji², Yan Li², Xiyue Cao³, Xiuling Shao⁴, Jianfei Xia⁵, Zonghua Wang⁶

Affiliations

¹ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Qingdao University, Qingdao, 266071, PR China; Technical Center of Qingdao Customs District, Qingdao, 266000, PR China.
² Technical Center of Qingdao Customs District, Qingdao, 266000, PR China.
³ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Qingdao University, Qingdao, 266071, PR China. Electronic address: caoxiyue@qdu.edu.cn.
⁴ Technical Center of Qingdao Customs District, Qingdao, 266000, PR China. Electronic address: ciqshao@163.com.
⁵ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Qingdao University, Qingdao, 266071, PR China. Electronic address: xiajianfei@126.com.
⁶ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Qingdao University, Qingdao, 266071, PR China.

PMID: 38743969
DOI: 10.1016/j.talanta.2024.126250

Abstract

In this research, self-screening aptamer and MOFs-derived nanomaterial have been combined to construct electrochemical aptasensor for environmental detection. By utilizing the large specific surface area of reduced graphene oxide (rGO), ZIF-8 was grown in situ on surface of rGO, and the composites was pyrolyzed to obtain MOFs-derived porous carbon materials (rGO-NCZIF). Thanks to the synergistic effect between rGO and NCZIF, the complex exhibits remarkable characteristics, including a high electron transfer rate and electrocatalytic activity. In addition, the orderly arrangement of imidazole ligands within ZIF-8 facilitated the uniform doping of nitrogen elements into the porous carbon, thereby significantly enhancing its electrochemical performance. After carboxylation, rGO-NCZIF was functionalized with self-screening aptamer for fabricating electrochemical aptasensor, which can be used to detect Erwinia cypripedii, a kind of quarantine plant bacteria, with detection limit of 4.92 × 10³ cfu/mL. Due to the simplicity and speed, the aptasensor is suitable for rapid customs inspection and quarantine. Additionally, the universality of this sensing strategy was verified through exosomes detection by changing the aptamer. The results indicated that the rGO-NCZIF-based electrochemical aptasensor had practical value in the environmental and medical fields.

Keywords: Autonomous screening aptamer; Electrochemical aptasensor; Erwinia cypripedii; Exosomes.