Higher diversity of sulfur-oxidizing bacteria based on soxB gene sequencing in surface water than in spring in Wudalianchi volcanic group, NE China

Lirong Geng; Lei Yang; Tao Liu; Shuang Zhang; Xindi Sun; Weidong Wang; Hong Pan; Lei Yan

doi:10.1007/s10123-024-00526-6

Higher diversity of sulfur-oxidizing bacteria based on soxB gene sequencing in surface water than in spring in Wudalianchi volcanic group, NE China

Int Microbiol. 2024 May 14. doi: 10.1007/s10123-024-00526-6. Online ahead of print.

Authors

Lirong Geng¹, Lei Yang¹, Tao Liu¹, Shuang Zhang¹, Xindi Sun¹, Weidong Wang^{1

2}, Hong Pan³, Lei Yan^{4

5}

Affiliations

¹ Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, China.
² Key Laboratory of Low‑carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs P. R. China, Heilongjiang Bayi Agricultural University, Daqing, 163319, China.
³ Institute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin, 150090, Heilongjiang, China.
⁴ Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, China. hekouyanlei@gmail.com.
⁵ Key Laboratory of Low‑carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs P. R. China, Heilongjiang Bayi Agricultural University, Daqing, 163319, China. hekouyanlei@gmail.com.

PMID: 38740654
DOI: 10.1007/s10123-024-00526-6

Abstract

Introduction: Sulfur-oxidizing bacteria (SOB) play a key role in the biogeochemical cycling of sulfur.

Objectives: To explore SOB diversity, distribution, and physicochemical drivers in five volcanic lakes and two springs in the Wudalianchi volcanic field, China.

Methods: This study analyzed microbial communities in samples via high-throughput sequencing of the soxB gene. Physical-chemical parameters were measured, and QIIME 2 (v2019.4), R, Vsearch, MEGA7, and Mothur processed the data. Alpha diversity indices and UPGMA clustering assessed community differences, while heat maps visualized intra-sample variations. Canoco 5.0 analyzed community-environment correlations, and NMDS, Adonis, and PcoA explored sample dissimilarities and environmental factor correlations. SPSS v.18.0 tested for statistical significance.

Results: The diversity of SOB in surface water was higher than in springs (more than 7.27 times). We detected SOB affiliated to β-proteobacteria (72.3 %), α-proteobacteria (22.8 %), and γ-proteobacteria (4.2 %) distributed widely in these lakes and springs. Rhodoferax and Cupriavidus were most frequent in all water samples, while Rhodoferax and Bradyrhizobium are dominant in surface waters but rare in springs. SOB genera in both habitats were positively correlated. Co-occurrence analysis identified Bradyrhizobium, Blastochloris, Methylibium, and Metyhlobacterium as potential keystone taxa. Redundancy analysis (RDA) revealed positive correlations between SOB diversity and total carbon (TC), Fe²⁺, and total nitrogen (TN) in all water samples.

Conclusion: The diversity and community structure of SOB in volcanic lakes and springs in the Wudalianchi volcanic group were clarified. Moreover, the diversity and abundance of SOB decreased with the variation of water openness, from open lakes to semi-enclosed lakes and enclosed lakes.

Keywords: soxB; Biodiversity; Sulfur-oxidizing bacteria; Waterbody types; Wudalianchi volcanic group.

Abstract

Grants and funding