Generation of induced pluripotent stem cells (NIMHi015-A) from a Parkinson's Disease patient harbouring a homozygous Exon 3 deletion in the PRKN gene

Roon Banerjee; Rituparna Ghanty; Soham Jagtap; Vikram Holla; Nitish Kamble; Ravi Yadav; Pramod Kumar Pal; Indrani Datta

doi:10.1016/j.scr.2024.103440

Generation of induced pluripotent stem cells (NIMHi015-A) from a Parkinson's Disease patient harbouring a homozygous Exon 3 deletion in the PRKN gene

Stem Cell Res. 2024 May 9:77:103440. doi: 10.1016/j.scr.2024.103440. Online ahead of print.

Authors

Roon Banerjee¹, Rituparna Ghanty¹, Soham Jagtap¹, Vikram Holla², Nitish Kamble², Ravi Yadav², Pramod Kumar Pal², Indrani Datta³

Affiliations

¹ Department of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, India.
² Department of Neurology, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, India.
³ Department of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, India. Electronic address: indrani@nimhans.kar.nic.in.

PMID: 38739971
DOI: 10.1016/j.scr.2024.103440

Abstract

The Parkin (PRKN) gene mutation is prevalent in young-onset Parkinson's disease (PD), typically emerging before age 30, accompanied by early motor symptoms. Induced pluripotent stem cells (iPSCs) were derived from peripheral blood mononuclear cells of a PD patient with an exon 3 deletion in PRKN using Sendai-virus reprogramming. PD diagnosis was confirmed via the Unified Parkinson's Disease Rating Scale (UPDRS). Characterization of the iPSC line ensured self-renewal and pluripotency. This resource serves as a valuable platform for drug screening and elucidating the pathophysiology of this mutation, facilitating advancements in PD research.