Hydrazo Pyrazole-Pyridone Fluorescent tag for NLO, Live cell imaging, LFPs visualization, Photophysical probing, and Electrochemical sensor for Dopamine detection

Luminescence. 2024 May;39(5):e4760. doi: 10.1002/bio.4760.

Abstract

The present communication reports on the synthesis of a novel methyl-pyridone azo fluorescent tag (MPAFT) were proven through 1H (NMR), FT-IR, UV-vis, and high-resolution mass spectrometry. The quantum chemical parameters of MPAFT were evaluated using density functional theory (DFT) analysis. It was further investigated for its latent fingerprint (LFPs) in various surfaces and anticounterfeiting applications. By exposing Level I-Level III, ridge features to UV light with a wavelength of 365 nm, a bioimaging investigation has also demonstrated the potential of MPAFT's emission behaviour. The cyclic voltammetry (CV) and linear sweep voltammetry (LSV) at MPAFT/MGCE (modified glassy carbon electrode) were used to explore the electrochemical sensitivity and reliable detection of dopamine (DA) in neutral PBS (pH 7) electrolyte solution, and the results show good sensitivity and detection. The lower detection limit for LSV was 0.81 μM under optimum conditions.

Keywords: azo colourant; computational study; dopamine sensing; latent fingerprints; live cell imaging.

MeSH terms

  • Density Functional Theory
  • Dopamine* / analysis
  • Dopamine* / chemistry
  • Electrochemical Techniques*
  • Fluorescent Dyes* / chemical synthesis
  • Fluorescent Dyes* / chemistry
  • Humans
  • Molecular Structure
  • Optical Imaging
  • Photochemical Processes
  • Pyrazoles* / chemistry
  • Pyridones* / chemistry

Substances

  • Pyridones
  • Dopamine
  • Fluorescent Dyes
  • Pyrazoles