High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression

Aohang Li; Jingxuan Wu

doi:10.1515/med-2023-0840

High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression

Open Med (Wars). 2024 May 9;19(1):20230840. doi: 10.1515/med-2023-0840. eCollection 2024.

Authors

Aohang Li¹, Jingxuan Wu²

Affiliations

¹ Clinical Laboratory Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
² Research Ward, Beijing Friendship Hospital, Capital Medical University, Xicheng District, Beijing, China.

Abstract

The aim of our study is to explore the mechanism of transcription-4 (STAT4) in acute myeloid leukemia (AML). STAT4 level in AML bone marrow samples/cells was analyzed using bioinformatics and quantitative real-time PCR. The correlation between high STAT4 expression and the prognosis of AML patients was analyzed. The viability, apoptosis, and angiogenesis of AML cells were detected. The levels of STAT4, vascular endothelial growth factor A (VEGFA), and apoptosis-related proteins (Bcl-2 and Bax) in transfected AML cells were examined. STAT4 level was upregulated in AML. STAT4 silencing decreased the viability and angiogenesis, yet increased the apoptosis of AML cells, while overexpressed STAT4 did conversely. VEGFA silencing counteracted the impacts of overexpressed STAT4 upon promoting viability and angiogenesis as well as repressing the apoptosis of AML cells. High STAT4 expression was correlated with poor prognosis of AML patients and facilitated disease progression via upregulating VEGFA expression.

Keywords: STAT4; VEGFA; acute myeloid leukemia; angiogenesis; apoptosis; poor prognosis.