Chemical fingerprinting and multicomponent quantitative analysis for quality control of Cinnamomum tamala collected from Western Himalaya by HPLC-DAD

Bibhuti Bhusan Champati; Prabhat Kumar Das; Chiranjibi Sahoo; Asit Ray; Sudipta Jena; Ambika Sahoo; Sanghamitra Nayak; Swaran Lata; Pratap Chandra Panda

doi:10.1016/j.heliyon.2024.e30361

Chemical fingerprinting and multicomponent quantitative analysis for quality control of Cinnamomum tamala collected from Western Himalaya by HPLC-DAD

Heliyon. 2024 Apr 25;10(9):e30361. doi: 10.1016/j.heliyon.2024.e30361. eCollection 2024 May 15.

Authors

Bibhuti Bhusan Champati¹, Prabhat Kumar Das¹, Chiranjibi Sahoo¹, Asit Ray¹, Sudipta Jena¹, Ambika Sahoo¹, Sanghamitra Nayak¹, Swaran Lata², Pratap Chandra Panda¹

Affiliations

¹ Centre for Biotechnology, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, 751 003, Odisha, India.
² ICFRE-Himalayan Forest Research Institute, Conifer Campus, Panthaghati, Shimla, 171 013, Himachal Pradesh, India.

Abstract

Cinnamomum tamala, commonly known as "Indian bay leaf" or "Tejpat", is an economically important plant widely used in medicine, food and cosmetic industries. Growing demand for its leaf and bark in the herbal trade and non-availability of quality materials lead to large-scale species admixture and adulteration in the global market. The present study aims at developing a validated HPLC-DAD (High-performance liquid chromatography coupled with diode array detection) method and multiple markers-based chemical fingerprints for quality evaluation of C. tamala leaf extracts. Five bioactive compounds, viz., coumarin, cinnamyl alcohol, cinnamic acid, cinnamaldehyde and cinnamyl acetate, were identified and quantified in 28 samples collected from the western Himalayan region of India. The chromatographic separation was achieved on Shimadzu Shimpak C18 column (dimension 250 × 4.6 mm, pore size 5 μm) with a gradient elution of mobile phase using acetonitrile and 0.1 percent phosphate buffer and the chromatograms were obtained at a wavelength of 265 nm. The method validation was done by analyzing the linearity, LOD, LOQ, precision, stability, repeatability and recovery rates of standard compounds for quantitative analysis. The values of coefficient of correlation (R²) were found to be close to 1 for linearity and similarity analysis; and standard deviation was less than 3 percent in case of precision, stability, repeatability and recovery rates. The content of target compounds such as coumarin, cinnamyl alcohol, cinnamic acid, cinnamaldehyde and cinnamyl acetate varied in the range of 0-1.09, 0-0.05, 0.07-0.51, 0.39-1.27 and 0-0.27 percent, respectively. In the chemical fingerprint of C. tamala leaves, a total of 13 peaks were assigned as common peaks. The results of the study indicated that the HPLC method now developed combining chemical fingerprint with quantification of analytes could serve as a useful tool for quality evaluation of herbal raw materials of C. tamala and a valuable reference for further study.

Keywords: Chemical fingerprint; Cinnamomum tamala and medicinal plant; HPLC; Herbal products; Quality control.