Functional properties, structural characteristics, and anti-complementary activities of two degraded polysaccharides from strawberry fruits

Mengyu Liu; Junwen Liu; Guoqiang Li; Dexin Zhang; Dong Qin; Libo Wang; Yaqin Xu

doi:10.1016/j.ijbiomac.2024.132263

Functional properties, structural characteristics, and anti-complementary activities of two degraded polysaccharides from strawberry fruits

Int J Biol Macromol. 2024 May 9;269(Pt 2):132263. doi: 10.1016/j.ijbiomac.2024.132263. Online ahead of print.

Authors

Mengyu Liu¹, Junwen Liu², Guoqiang Li¹, Dexin Zhang¹, Dong Qin³, Libo Wang⁴, Yaqin Xu⁵

Affiliations

¹ College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.
² Feixian Forestry Development Center, Linyi, Shandong 273400, China.
³ College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
⁴ College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address: wanglibo@neau.edu.cn.
⁵ College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address: xuyaqin@neau.edu.cn.

PMID: 38734332
DOI: 10.1016/j.ijbiomac.2024.132263

Abstract

Two low-molecular-weight polysaccharides (DPSP50 and DPSP70) were obtained using hydrogen peroxide-vitamin C (H₂O₂-Vc) treatment at 50 °C and 70 °C, respectively. Both DPSP50 and DPSP70 comprised the same six monosaccharides in different ratios, and their molecular weights (M_ws) were 640 kDa and 346 kDa, respectively. Functional properties analyses demonstrated that DPSP50 and DPSP70 each had an excellent water holding capacity, oil absorption capacity, and emulsion properties, as well as shear-thinning characteristics and viscoelastic properties. Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic assays confirmed the existence of α-, β-pyranose rings and the same six sugar residues in DPSP50 and DPSP70. The results of Congo red test, scanning electron microscopy (SEM), and X-ray diffraction (XRD) demonstrated that DPSP50 and DPSP70 did not contain triple-helix conformations, but were amorphous aggregates with flake-like shape and rough surface. Additionally, both DPSP50 and DPSP70 showed strong anti-complementary activities through the classical pathway and the alternative pathway. The results support the potential utility of these degraded polysaccharides from strawberry fruits in functional foods and medicines.

Keywords: Anti-complementary activity; Degraded polysaccharides; Functional properties; Strawberry fruits; Structure characteristic.