Pt-S bond stabilized DNAzyme nanosensor with thiol-resistance enabling high-fidelity biosensing

Yanli Lei; Xinyan He; Yu Zeng; Xinyu Wang; Le Yang; Xiaowen Liu; Zhihe Qing

doi:10.1016/j.talanta.2024.126187

Pt-S bond stabilized DNAzyme nanosensor with thiol-resistance enabling high-fidelity biosensing

Talanta. 2024 Apr 29:276:126187. doi: 10.1016/j.talanta.2024.126187. Online ahead of print.

Authors

Yanli Lei¹, Xinyan He¹, Yu Zeng¹, Xinyu Wang¹, Le Yang¹, Xiaowen Liu², Zhihe Qing³

Affiliations

¹ Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, School of Food Science and Bioengineering, Changsha University of Science and Technology, Changsha, 410004, China.
² Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University, Changsha, 410083, China. Electronic address: 124821877@qq.com.
³ Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, School of Food Science and Bioengineering, Changsha University of Science and Technology, Changsha, 410004, China. Electronic address: qingzhihe@hnu.edu.cn.

PMID: 38733933
DOI: 10.1016/j.talanta.2024.126187

Abstract

Gold nanoparticles (Au NPs) have been widely utilized in developing DNAzyme-functionalized nanosensors, most of which were engineered by attaching the thiolated DNAzymes to Au NPs via Au-S bonding. However, the Au NP-DNAzyme nanosensors always suffer from signal distortion when applied in complex environment with abundant thiols, which poses challenge for practical applications. Here, we focus on addressing the root cause of the issue and propose to decorate the Au NPs with a thin layer of platinum, thus facilitating the conjugation of DNAzymes through Pt-S bonding, a thiol-resistant cross-linking. The Pt-S bond stabilized DNAzyme nanosensor effectively minimized false positive signals when detecting l-histidine in infant formulas, as compared to the Au-S stabilized counterpart. This innovative strategy holds promise for high-fidelity biosensing, improving the practical applicability of Au NP-based DNAzyme nanosensor.

Keywords: Biosensor; DNAzyme; Gold nanoparticle; Platinum; Thiol.