Detection of Dopamine Based on Aptamer-Modified Graphene Microelectrode

Sensors (Basel). 2024 May 5;24(9):2934. doi: 10.3390/s24092934.

Abstract

In this paper, a novel aptamer-modified nitrogen-doped graphene microelectrode (Apt-Au-N-RGOF) was fabricated and used to specifically identify and detect dopamine (DA). During the synthetic process, gold nanoparticles were loaded onto the active sites of nitrogen-doped graphene fibers. Then, aptamers were modified on the microelectrode depending on Au-S bonds to prepare Apt-Au-N-RGOF. The prepared microelectrode can specifically identify DA, avoiding interference with other molecules and improving its selectivity. Compared with the N-RGOF microelectrode, the Apt-Au-N-RGOF microelectrode exhibited higher sensitivity, a lower detection limit (0.5 μM), and a wider linear range (1~100 μM) and could be applied in electrochemical analysis fields.

Keywords: aptamers; electrochemistry; graphene fibers; microelectrodes; specific recognition.

MeSH terms

  • Aptamers, Nucleotide* / chemistry
  • Biosensing Techniques / methods
  • Dopamine* / analysis
  • Dopamine* / chemistry
  • Electrochemical Techniques* / methods
  • Gold* / chemistry
  • Graphite* / chemistry
  • Limit of Detection
  • Metal Nanoparticles* / chemistry
  • Microelectrodes*
  • Nitrogen / chemistry

Substances

  • Graphite
  • Dopamine
  • Aptamers, Nucleotide
  • Gold
  • Nitrogen