The effects of exposure to and timing of a choline-deficient diet during pregnancy and early postnatal life on the skeletal muscle transcriptome of the offspring

Joanna Mikołajczyk-Stecyna; Ewelina Zuk; Agata Chmurzynska; Malgorzata Blatkiewicz; Karol Jopek; Marcin Rucinski

doi:10.1016/j.clnu.2024.05.002

The effects of exposure to and timing of a choline-deficient diet during pregnancy and early postnatal life on the skeletal muscle transcriptome of the offspring

Clin Nutr. 2024 May 7;43(6):1503-1515. doi: 10.1016/j.clnu.2024.05.002. Online ahead of print.

Authors

Joanna Mikołajczyk-Stecyna¹, Ewelina Zuk², Agata Chmurzynska², Malgorzata Blatkiewicz³, Karol Jopek³, Marcin Rucinski³

Affiliations

¹ Poznań University of Life Sciences, Department of Human Nutrition and Dietetics, Poznań, Poland. Electronic address: joanna.stecyna@up.poznan.pl.
² Poznań University of Life Sciences, Department of Human Nutrition and Dietetics, Poznań, Poland.
³ Poznań University of Medical Sciences, Department of Histology and Embryology, Poznań, Poland.

PMID: 38729079
DOI: 10.1016/j.clnu.2024.05.002

Abstract

Background & aims: Nonalcoholic fatty liver disease (NAFLD) is related to muscle loss, but the precise mechanism underlying this association remains unclear. The aim of the present study was thus to determine the influence of maternal fatty liver and dietary choline deficiency during pregnancy and/or lactation periods on the skeletal muscle gene expression profile among 24-day-old male rat offspring.

Methods: Histological examination of skeletal muscle tissue specimens obtained from offspring of dams suffering from fatty liver, provided with proper choline intake during pregnancy and lactation (NN), fed a choline-deficient diet during both periods (DD), deprived of choline only during pregnancy (DN), or only during lactation (ND), was performed. The global transcriptome pattern was assessed using a microarray approach (Affymetrix® Rat Gene 2.1 ST Array Strip). The relative expression of selected genes was validated by real-time PCR (qPCR).

Results: Morphological differences in fat accumulation in skeletal muscle related to choline supply were observed. The global gene expression profile was consistent with abnormal morphological changes. Mettl21c gene was overexpressed in all choline-deficient groups compared to the NN group, while two genes, Cdkn1a and S100a4, were downregulated. Processes of protein biosynthesis were upregulated, and processes related to cell proliferation and lipid metabolism were inhibited in DD, DN, and ND groups compared to the NN group.

Conclusions: Prenatal and early postnatal exposure to fatty liver and dietary choline deficiency leads to changes in the transcriptome profile in skeletal muscle of 24-day old male rat offspring and is associated with muscle damage, but the mechanism of it seems to be different at different developmental stages of life. Adequate choline intake during pregnancy and lactation can prevent severe muscle disturbance in the progeny of females suffering from fatty liver.

Keywords: Choline; Fatty liver; Prenatal programing; Skeletal muscle; Suckling period; Transcriptome profile.