68Ga-Fibroblast Activation Protein Inhibitor PET/CT Improves Detection of Intermediate and Low-Grade Sarcomas and Identifies Candidates for Radiopharmaceutical Therapy

Helena Lanzafame; Ilektra A Mavroeidi; Kim M Pabst; Mélanie Desaulniers; Marc Ingenwerth; Nader Hirmas; Lukas Kessler; Michael Nader; Timo Bartel; Stephan Leyser; Francesco Barbato; Martin Schuler; Sebastian Bauer; Jens T Siveke; Ken Herrmann; Rainer Hamacher; Wolfgang P Fendler

doi:10.2967/jnumed.123.267248

⁶⁸Ga-Fibroblast Activation Protein Inhibitor PET/CT Improves Detection of Intermediate and Low-Grade Sarcomas and Identifies Candidates for Radiopharmaceutical Therapy

J Nucl Med. 2024 May 9:jnumed.123.267248. doi: 10.2967/jnumed.123.267248. Online ahead of print.

Authors

Helena Lanzafame^{1

2}, Ilektra A Mavroeidi^{2

3}, Kim M Pabst^{4

2}, Mélanie Desaulniers^{4

2

5}, Marc Ingenwerth^{3

6}, Nader Hirmas^{4

2}, Lukas Kessler^{4

2

7}, Michael Nader^{4

2}, Timo Bartel^{4

2}, Stephan Leyser^{4

2}, Francesco Barbato^{4

2}, Martin Schuler^{2

8}, Sebastian Bauer^{2

3

8}, Jens T Siveke^{2

8

9}, Ken Herrmann^{4

2

7}, Rainer Hamacher^{2

3}, Wolfgang P Fendler^{4

2}

Affiliations

¹ Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany; helena.lanzafame@uk-essen.de.
² Cancer Consortium partner site Essen/Düsseldorf, DKFZ and University Hospital Essen, Essen, Germany.
³ Department of Medical Oncology, West German Cancer Center, University Hospital Essen, Essen, Germany.
⁴ Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany.
⁵ Department of Nuclear Medicine and Radiobiology, Université de Sherbrooke, Sherbrooke, Québec, Canada.
⁶ Institute of Pathology, University Hospital Essen, Essen, Germany.
⁷ Institute of Diagnostic and Interventional Radiology and Neuroradiology, University Hospital Essen, Essen, Germany.
⁸ National Center for Tumor Diseases West, Campus Essen, Essen, Germany; and.
⁹ Bridge Institute of Experimental Tumor Therapy and Division of Solid Tumor Translational Oncology, West German Cancer Center, University Hospital Essen, Essen, Germany.

PMID: 38724279
DOI: 10.2967/jnumed.123.267248

Abstract

Fibroblast activation protein-α (FAP) is often highly expressed by sarcoma cells and by sarcoma-associated fibroblasts in the tumor microenvironment. This makes it a promising target for imaging and therapy. The level of FAP expression and the diagnostic value of ⁶⁸Ga-FAP inhibitor (FAPI) PET for sarcoma subtypes are unknown. We assessed the diagnostic performance and accuracy of ⁶⁸Ga-FAPI PET in various bone and soft-tissue sarcomas. Potential eligibility for FAP-targeted radiopharmaceutical therapy (FAP-RPT) was evaluated. Methods: This prospective observational trial enrolled 200 patients with bone and soft-tissue sarcoma who underwent ⁶⁸Ga-FAPI PET/CT and ¹⁸F-FDG PET/CT (186/200, or 93%) for staging or restaging. The number of lesions detected and the uptake (SUV_max) of the primary tumor, lymph nodes, and visceral and bone metastases were analyzed. The Wilcoxon test was used for semiquantitative assessment. The association of ⁶⁸Ga-FAPI uptake intensity, histopathologic grade, and FAP expression in sarcoma biopsy samples was analyzed using Spearman r correlation. The impact of ⁶⁸Ga-FAPI PET on clinical management was investigated using questionnaires before and after PET/CT. Eligibility for FAP-RPT was defined by an SUV_max greater than 10 for all tumor regions. Results: ⁶⁸Ga-FAPI uptake was heterogeneous among sarcoma subtypes. The 3 sarcoma entities with the highest uptake (mean SUV_max ± SD) were solitary fibrous tumor (24.7 ± 11.9), undifferentiated pleomorphic sarcoma (18.8 ± 13.1), and leiomyosarcoma (15.2 ± 10.2). Uptake of ⁶⁸Ga-FAPI versus ¹⁸F-FDG was significantly higher in low-grade sarcomas (10.4 ± 8.5 vs. 7.0 ± 4.5, P = 0.01) and in potentially malignant intermediate or unpredictable sarcomas without a World Health Organization grade (not applicable [NA]; 22.3 ± 12.5 vs. 8.5 ± 10.0, P = 0.0004), including solitary fibrous tumor. The accuracy, as well as the detection rates, of ⁶⁸Ga-FAPI was higher than that of ¹⁸F-FDG in low-grade sarcomas (accuracy, 92.2 vs. 80.0) and NA sarcomas (accuracy, 96.9 vs. 81.9). ⁶⁸Ga-FAPI uptake and the histopathologic FAP expression score (n = 89) were moderately correlated (Spearman r = 0.43, P < 0.0002). Of 138 patients, 62 (45%) with metastatic sarcoma were eligible for FAP-RPT. Conclusion: In patients with low-grade and NA sarcomas, ⁶⁸Ga-FAPI PET demonstrates uptake, detection rates, and accuracy superior to those of ¹⁸F-FDG PET. ⁶⁸Ga-FAPI PET criteria identified eligibility for FAP-RPT in about half of sarcoma patients.

Keywords: 68Ga-FAPI PET; cancer imaging; fibroblast activation protein; sarcoma; theranostic.