UGT1A1*28 detection using high-resolution agarose gel electrophoresis

Shirou Tsuchida; Takaaki Hirayama; Hayato Nunose; Hinako Suzuki; Ryo Hakota; Tsugumi Shindo; Koji Nakagawa

doi:10.1016/j.heliyon.2024.e30465

UGT1A128* detection using high-resolution agarose gel electrophoresis

Heliyon. 2024 Apr 27;10(9):e30465. doi: 10.1016/j.heliyon.2024.e30465. eCollection 2024 May 15.

Authors

Shirou Tsuchida¹, Takaaki Hirayama¹, Hayato Nunose¹, Hinako Suzuki¹, Ryo Hakota¹, Tsugumi Shindo¹, Koji Nakagawa¹

Affiliation

¹ School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu-cho, Ishikari-gun, Hokkaido, 061-0293, Japan.

Abstract

A new UGT1A1*28 detection method combining PCR and high-resolution agarose gel electrophoresis was developed. The viability of this method was demonstrated on 15 healthy adult volunteers. Subjects included 13 wild type homozygotes (86.7 %), 2 heterozygotes (13.3 %), and no mutant type homozygotes (0 %). The new UGT1A1*28 detection method results were fully consistent with DNA sequencing. PCR and agarose gel electrophoresis are common techniques with high-resolution agarose gels available commercially. These results support the clinical viability of this method potentially reducing UGT1A1*28 diagnosis complexity and cost.

Keywords: Agarose gel electrophoresis; Genotyping; PCR; UGT1A1*28.