Draft genome sequence and morphological data of Planifilum fimeticola PLACP1, a thermophilic chloramphenicol-resistant bacterium isolated from thermophilic sludge

Hou-Chia Tseng; Minenosuke Matsutani; Naoshi Fujimoto; Akihiro Ohnishi

doi:10.1016/j.dib.2024.110447

Draft genome sequence and morphological data of Planifilum fimeticola PLACP1, a thermophilic chloramphenicol-resistant bacterium isolated from thermophilic sludge

Data Brief. 2024 Apr 18:54:110447. doi: 10.1016/j.dib.2024.110447. eCollection 2024 Jun.

Authors

Hou-Chia Tseng¹, Minenosuke Matsutani², Naoshi Fujimoto¹, Akihiro Ohnishi¹

Affiliations

¹ Department of Fermentation Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1 Sakuragaoka 1-chome, Setagaya-ku, Tokyo 156-8502, Japan.
² NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo 156-8502, Japan.

Abstract

Planifilum is a thermophilic aerobic Actinomyces often found in compost that is suggested to play a primary role in the degradation of organic matter and is a potential antibiotic-resistance gene (ARG)-hosting bacterium during the composting process. Planifilum fimeticola PLACP1 was isolated from thermophilic sludge on a Columbia plate supplemented with chloramphenicol. PLACP1 was Gram-stain-positive with cells longer than 20 μm that branched and intertwined with each other. A draft genome sequence of P. fimeticola PLACP1 was generated using the Illumina NovaSeq system and deposited in the National Center for Biotechnology Information database under the BioProject accession numbers PRJDB17484 and SAMD00736731. The genome sequence comprised 3,395,140 bp, with 57.97 % GC content and 3,368 genes, including 3,267 protein-coding, 6 rRNA, and 56 tRNA genes. Based on the Comprehensive Antibiotic Resistance Database, 237 predicted gene products were related to ARGs, including 44 macrolide antibiotic-related genes (19 %) as the largest group. This dataset will be beneficial for the morphological identification, comparative genomic analyses, and ARG research in the genus Planifilum.

Keywords: Antibiotics; Compost; Genomic analysis; Thermophilic sludge.