Rapid and specific detection of thiabendazole: enzymatic digestion-enabled fluorescent aptasensor

Abstract

Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R²) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices.

Keywords: Biosensing; Enzymatic digestion; Fluorescence spectroscopy; Oligonucleotide switching structures; Thiabendazole.