Perturbed maternal microbiota shapes offspring microbiota during early colonization period in mice

Shiro Tochitani; Takamitsu Tsukahara; Ryo Inoue

doi:10.2183/pjab.100.020

Perturbed maternal microbiota shapes offspring microbiota during early colonization period in mice

Proc Jpn Acad Ser B Phys Biol Sci. 2024 May 2. doi: 10.2183/pjab.100.020. Online ahead of print.

Authors

Shiro Tochitani^{1

2

3

4}, Takamitsu Tsukahara⁵, Ryo Inoue⁶

Affiliations

¹ Graduate School of Health Science, Suzuka University of Medical Science, Suzuka, Mie, Japan.
² Faculty of Health Science, Suzuka University of Medical Science, Suzuka, Mie, Japan.
³ Division of Development of Mental Functions, Research Center for Child Mental Development, University of Fukui, Fukui, Japan.
⁴ Department of Child Development, United Graduate School of Child Development, Osaka University, Osaka, Japan.
⁵ Kyoto Institute of Nutrition & Pathology, Kyoto, Japan.
⁶ Laboratory of Animal Science, Department of Applied Biological Sciences, Faculty of Agriculture, Setsunan University, Hirakata, Osaka, Japan.

PMID: 38692912
DOI: 10.2183/pjab.100.020

Abstract

Recent studies have highlighted the impact of disrupted maternal gut microbiota on the colonization of offspring gut microbiota, with implications for offspring developmental trajectories. The extent to which offspring inherit the characteristics of altered maternal gut microbiota remains elusive. In this study, we employed a mouse model where maternal gut microbiota disruption was induced using non-absorbable antibiotics. Systematic chronological analyses of dam fecal samples, offspring luminal content, and offspring gut tissue samples revealed a notable congruence between offspring gut microbiota profiles and those of the perturbed maternal gut microbiota, highlighting the profound influence of maternal microbiota on early-life colonization of offspring gut microbiota. Nonetheless, certain dominant bacterial genera in maternal microbiota did not transfer to the offspring, indicating a bacterial taxonomy-dependent mechanism in the inheritance of maternal gut microbiota. Our results embody the vertical transmission dynamics of disrupted maternal gut microbiota in an animal model, where the gut microbiota of an offspring closely mirrors the gut microbiota of its mother.

Keywords: DOHaD; luminal bacteria; mucosa-associated bacteria; perturbed maternal gut microbiota (PMGM) model; priority effect of bacterial colonization; transgenerational transmission.