Growth Suppression of Oral Squamous Cell Carcinoma Cells by Lactobacillus Acidophilus

Adel Al-Asfour; Radhika G Bhardwaj; Maribasappa Karched

doi:10.1016/j.identj.2024.03.017

Growth Suppression of Oral Squamous Cell Carcinoma Cells by Lactobacillus Acidophilus

Int Dent J. 2024 Apr 27:S0020-6539(24)00103-5. doi: 10.1016/j.identj.2024.03.017. Online ahead of print.

Authors

Adel Al-Asfour¹, Radhika G Bhardwaj², Maribasappa Karched³

Affiliations

¹ Department of Surgical Sciences, College of Dentistry, Kuwait University, Kuwait City, Kuwait.
² Oral Microbiology Research Laboratory, Department of Bioclinical Sciences, College of Dentistry, Kuwait University, Kuwait City, Kuwait.
³ Oral Microbiology Research Laboratory, Department of Bioclinical Sciences, College of Dentistry, Kuwait University, Kuwait City, Kuwait. Electronic address: Maribasappa.karched@ku.edu.kw.

PMID: 38679518
DOI: 10.1016/j.identj.2024.03.017

Abstract

Objectives: Oral squamous cell carcinoma (OSCC) is a highly aggressive form of oral cancer. Probiotic lactobacilli have demonstrated anticancer effects, whilst their interaction with Streptococcus mutans in this context remains unexplored. The objective of this study was to investigate the antiproliferative effect of Lactobacillus acidophilus on OSCC and to understand the effect of S mutans on OSCCs and whether it affects the antiproliferative potential of L acidophilus when co-exposed to OSCC.

Methods: The human head and neck squamous cell carcinoma cells of the oral cavity (HNO97 cell line) were exposed to cultures of L acidophilus and S mutans separately and in combination. Further, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to assess the viability of HNO97 cells. Bacterial adhesion to HNO97 cells was examined by confocal microscopy and apoptosis by Nexin staining. To understand the underlying mechanism of apoptosis, expression of the tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) gene and protein were determined by real-time polymerase chain reaction and quantitative enzyme-linked immunosorbent assay, respectively.

Results: A significant decrease (53%-56%) in the viability of HNO97 cells on exposure to L acidophilus, S mutans, and the 2 species together demonstrated the antiproliferative activity of L acidophilus and S mutans. Both bacteria showed adhesion to HNO97 cells. The expression of the TRAIL gene increased 5-fold in HNO97 cells on treatment with L acidophilus and S mutans, which further increased to ∼17-fold with both species present. Expression levels of the TRAIL protein were significantly (P < .05) increased in bacteria-treated cell lysates. Further, bacteria-treated HNO97 cells exhibited lower live and intact cell percentages with higher proportions of cells in early and late apoptotic stages.

Conclusions: L acidophilus exhibits the antiproliferative activity against OSCC cells possibly partially via a TRAIL-induced mechanism of apoptosis, which is not affected by the presence of S mutans. These findings may encourage further investigation into the possible therapeutic application of probiotic L acidophilus in OSCC.

Keywords: Apoptosis; Lactobacillus acidophilus; Oral cancer; Oral squamous cell carcinoma; Probiotics; Streptococcus mutans; TNF-related apoptosis inducing ligand.