Multi-scale perceptual YOLO for automatic detection of clue cells and trichomonas in fluorescence microscopic images

Abstract

Vaginitis is a common disease among women and has a high recurrence rate. The primary diagnosis method is fluorescence microscopic inspection, but manual inspection is inefficient and can lead to false detection or missed detection. Automatic cell identification and localization in microscopic images are necessary. For vaginitis diagnosis, clue cells and trichomonas are two important indicators and are difficult to be detected because of the different scales and image characteristics. This study proposes a Multi-Scale Perceptual YOLO (MSP-YOLO) with super-resolution reconstruction branch to meet the detection requirements of clue cells and trichomonas. Based on the scales and image characteristics of clue cells and trichomonas, we employed a super-resolution reconstruction branch to the detection network. This branch guides the detection branch to focus on subtle feature differences. Simultaneously, we proposed an attention-based feature fusion module that is injected with dilated convolutional group. This module makes the network pay attention to the non-centered features of the large target clue cells, which contributes to the enhancement of detection sensitivity. Experimental results show that the proposed detection network MSP-YOLO can improve sensitivity without compromising specificity. For clue cell and trichomoniasis detection, the proposed network achieved sensitivities of 0.706 and 0.910, respectively, which were 0.218 and 0.051 higher than those of the baseline model. In this study, the characteristics of the super-resolution reconstruction task are used to guide the network to effectively extract and process image features. The novel proposed network has an increased sensitivity, which makes it possible to detect vaginitis automatically.

Keywords: Cell detection; Multi-scale; Spatial attention; Super-resolution reconstruction; Vaginosis.