Orthotopic MC-38 Allograft as a Robust Preclinical Model of Colorectal Carcinoma

Marina H Yakou; Shoukat Afshar-Sterle; Matthias Ernst; Lisa A Mielke

doi:10.1007/978-1-0716-3858-3_14

Orthotopic MC-38 Allograft as a Robust Preclinical Model of Colorectal Carcinoma

Methods Mol Biol. 2024:2806:197-207. doi: 10.1007/978-1-0716-3858-3_14.

Authors

Marina H Yakou¹, Shoukat Afshar-Sterle², Matthias Ernst³, Lisa A Mielke⁴

Affiliations

¹ Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, Australia. marina.yakou@onjcri.org.au.
² Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, Australia.
³ Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, Australia. matthias.ernst@onjcri.org.au.
⁴ Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, Australia. lisa.mielke@onjcri.org.au.

PMID: 38676804
DOI: 10.1007/978-1-0716-3858-3_14

Abstract

Colorectal cancer (CRC) is a significant global health concern, requiring effective preclinical models for studying its development and testing therapies. Mouse models have been used, including spontaneous tumors, carcinogen exposure, and tumor cell implantation as xenografts or at orthotopic sites. Here, we describe an orthotopic preclinical model of CRC, which provides a valuable tool for studying tumor growth and the tumor microenvironment, offering a more accurate representation of human CRC compared to xenograft models.

Keywords: Cancer; Colonoscopy; Colorectal cancer; Colorectal carcinoma; Orthotopic MC-38 allograft.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Allografts
Animals
Cell Line, Tumor
Colorectal Neoplasms* / pathology
Disease Models, Animal*
Humans
Mice
Tumor Microenvironment