Fumaric acid production by Rhizopus species from acid hydrolysate of oil palm empty fruit bunches

Omar D Pairazamán; Adenise L Woiciechowski; Luis A Zevallos; Valcineide O Andrade Tanobe; Arion Zandona; Carlos R Soccol

doi:10.1007/s42770-024-01322-0

Fumaric acid production by Rhizopus species from acid hydrolysate of oil palm empty fruit bunches

Braz J Microbiol. 2024 Apr 26. doi: 10.1007/s42770-024-01322-0. Online ahead of print.

Authors

Omar D Pairazamán^{1

2

3}, Adenise L Woiciechowski^{4

5}, Luis A Zevallos¹, Valcineide O Andrade Tanobe⁶, Arion Zandona^{1

7}, Carlos R Soccol^{1

7}

Affiliations

¹ Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Brazil, Polytechnic Center, CP 19011, Curitiba, 81531-908, Brazil.
² Biological Science Department, National University of Cajamarca, Cajamarca, Peru.
³ Bacteriology Laboratory, Regional Public Health Laboratory, Cajamarca, Peru.
⁴ Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Brazil, Polytechnic Center, CP 19011, Curitiba, 81531-908, Brazil. adenise@ufpr.br.
⁵ Chemical Engineering Department, Federal University of Paraná, Brazil, Polytechnic Center, CP 19011, Curitiba, 81531-908, Brazil. adenise@ufpr.br.
⁶ Division of Industrial Electromechanics, Technological University of Jalisco, Guadalajara, Mexico.
⁷ Chemical Engineering Department, Federal University of Paraná, Brazil, Polytechnic Center, CP 19011, Curitiba, 81531-908, Brazil.

PMID: 38671219
DOI: 10.1007/s42770-024-01322-0

Abstract

The hemicellulosic fraction of lignocellulosic biomass is a very important material, due to the significant concentration of pentoses present in its composition and that can be used sustainably in biotechnological processes such as the production of fumaric acid. Research efforts are currently being promoted for the proper disposal and valorization of empty fruit bunches (EFB) from oil palm. In this work, seventeen Rhizopus species were evaluated in a fermentation medium with EFB hydrolyzate, without detoxification, as a carbon source for fumaric acid production. Rhizopus circicans 1475 and Rhizopus 3271 achieved productions of 5.65 g.L^-1 and 5.25 g.L^-1 of fumaric acid at 30 °C, 120 rpm for 96 h, respectively. The percentage of consumed sugars, mainly pentoses, was 24.88% and 34.02% for R. circicans 1475 and R 3271, respectively. Soy peptone and ammonium sulfate were evaluated as nitrogen sources, where soy peptone stimulated the formation of biomass pellets while ammonium sulfate produced mycelia and clamps.

Keywords: Rhizopus circicans; Acid treatment; EFB; Fumaric acid; Pentoses.