Enzyme-integrated metal-organic framework platform for cascade detection of α-amylase

Nana Li; Liangqiang Chen; Wanqiu Huang; Mengdi Hao; Huabin Tu; Hao Shen; Fan Yang; Shaoning Yu

doi:10.1016/j.ijbiomac.2024.131870

Enzyme-integrated metal-organic framework platform for cascade detection of α-amylase

Int J Biol Macromol. 2024 Apr 24;268(Pt 1):131870. doi: 10.1016/j.ijbiomac.2024.131870. Online ahead of print.

Authors

Nana Li¹, Liangqiang Chen², Wanqiu Huang², Mengdi Hao¹, Huabin Tu², Hao Shen³, Fan Yang⁴, Shaoning Yu⁵

Affiliations

¹ Institute of Mass Spectrometry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
² Kweichow Moutai Group, Renhuai, Guizhou 564501, China; Guizhou Key Laboratory of Microbial Resources Exploration in Fermentation industry, Kweichow Moutai Group, Zunyi 564501, China.
³ Institute of Mass Spectrometry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China. Electronic address: shenhao@nbu.edu.cn.
⁴ Kweichow Moutai Group, Renhuai, Guizhou 564501, China. Electronic address: yangfanmt@189.cn.
⁵ Institute of Mass Spectrometry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China. Electronic address: yushaoning@nbu.edu.cn.

PMID: 38670199
DOI: 10.1016/j.ijbiomac.2024.131870

Abstract

As one of the most important industrial enzymes, α-amylase is widely used in food processing, such as starch sugar and fermentation, bringing high added value to industry of more than a trillion dollars. We developed a multi-enzyme system (Glu&Gox@Cu-MOF-74) prepared by embedding α-glucosidase (Glu) and glucose oxidase (Gox) into the biomimetic metal-organic framework Cu-MOF-74 using in situ encapsulation within 15 min at room temperature for efficient and sensitive detection of α-amylase activity. Benefitting from the remarkable peroxidase-mimicking property and rigid skeleton of Cu-MOF-74, the biocatalytic platform exhibited excellent cascade activity and tolerance in various extremely harsh environments compared to natural enzymes. On this basis, a cascade biocatalytic platform was constructed for the detection of α-amylase activity with wide linear range (5-100 U/L) and low limit of detection (1.45 U/L). The colorimetric cascade scheme is important for the sensitive and selective determination of α-amylase in complex fermentation samples, and the detection time is short (∼0.5 h). This work provides new ideas for the detection of α-amylase based on the cascade amplification method.

Keywords: Cascade catalysis; Enzyme immobilization; Metal-organic framework; α-Amylase detection.