We report a series of ethenylene-bridged D-π-A BODIPY-xanthene hybrid dyes with precisely regulated absorption bands ranging from the far-red to the near-infrared region (NIR, 700-1000 nm) through rational molecular design. These dyes have excellent photoacoustic properties, and their biocompatibility can be significantly improved by facilely introducing water-soluble groups. In vivo two-channel multiplexed photoacoustic imaging demonstrated their high-resolution imaging capabilities, making them promising candidates for future NIR bioimaging applications.