The Plasmodiophora brassicae Golgi-localized UPF0016 protein PbGDT1 mediates calcium but not manganese transport in yeast and Nicotiana benthamiana

Md Musharaf Hossain; Cresilda Vergara Alinapon; Christopher D Todd; Yangdou Wei; Peta C Bonham-Smith

doi:10.1016/j.fgb.2024.103896

The Plasmodiophora brassicae Golgi-localized UPF0016 protein PbGDT1 mediates calcium but not manganese transport in yeast and Nicotiana benthamiana

Fungal Genet Biol. 2024 Apr 24:172:103896. doi: 10.1016/j.fgb.2024.103896. Online ahead of print.

Authors

Md Musharaf Hossain¹, Cresilda Vergara Alinapon¹, Christopher D Todd¹, Yangdou Wei¹, Peta C Bonham-Smith²

Affiliations

¹ Department of Biology, University of Saskatchewan, Saskatoon S7N5E2, Saskatchewan, Canada.
² Department of Biology, University of Saskatchewan, Saskatoon S7N5E2, Saskatchewan, Canada. Electronic address: peta.bonhams@usask.ca.

PMID: 38663635
DOI: 10.1016/j.fgb.2024.103896

Abstract

Manganese and calcium homeostasis and signalling, in eukaryotic organisms, are regulated through membrane located pumps, channels and exchangers, including the Mn²⁺/Ca²⁺ uncharacterized protein family 0016 (UPF0016). Here we show that Plasmodiophora brassicae PbGDT1 is a member of the UPF0016 and an ortholog of Saccharomyces cerevisiae Gdt1p (GCR Dependent Translation Factor 1) protein involved in manganese homeostasis as well as the calcium mediated stress response in yeast. PbGDT1 complemented the ScGdt1p and ScPMR1 (Ca²⁺ ATPase) double null mutant under elevated calcium stress but not under elevated manganese conditions. In both yeast and Nicotiana benthamiana, PbGDT1 localizes to the Golgi apparatus, with additional ER association in N. benthamiana. Expression of PbGDT1 in N. benthamiana, suppresses BAX-triggered cell death, further highlighting the importance of calcium homeostasis in maintaining cell physiology and integrity in a stress environment.

Keywords: Calcium homeostasis; Gdt1 family; Golgi apparatus; Protist; Transporter.