Dual-mode aptasensor based on P-CeO2NR@Mxene and exonuclease I-assisted target recycling for malachite green detection

Mingyue Ye; Xianfeng Lin; Jin Li; Xiaowan Chen; Dichen Ying; Shijia Wu; Zhouping Wang; Nuo Duan

doi:10.1016/j.foodchem.2024.139399

Dual-mode aptasensor based on P-CeO₂NR@Mxene and exonuclease I-assisted target recycling for malachite green detection

Food Chem. 2024 Apr 17:451:139399. doi: 10.1016/j.foodchem.2024.139399. Online ahead of print.

Authors

Mingyue Ye¹, Xianfeng Lin¹, Jin Li¹, Xiaowan Chen¹, Dichen Ying¹, Shijia Wu², Zhouping Wang², Nuo Duan³

Affiliations

¹ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
³ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China. Electronic address: duannuo@jiangnan.edu.cn.

PMID: 38663240
DOI: 10.1016/j.foodchem.2024.139399

Abstract

Malachite green (MG) has been illicitly employed in aquaculture as a parasiticide, however, its teratogenic and carcinogenic effects pose a significant human health threat. Herein, a dual-mode colorimetric and electrochemical aptasensor was fabricated for MG detection, capitalizing on the robust catalytic and peroxidase-like activity of P-CeO₂NR@Mxene and good capture efficiency of a tetrahedral DNA nanostructure (TDN) designed with multiple aptamers (m-TDN). P-CeO₂NR@Mxene-modified complementary DNA (cDNA) served as both colorimetric and electrochemical probe. m-TDN was attached to AuE to capture MG and P-CeO₂NR@Mxene/cDNA. The superior aptamer and MG binding to cDNA regulated signals and enabled precise MG quantification. The further introduced Exo I enabled aptamer hydrolysis, releasing MG for further binding rounds, allowing target recycling amplification. Under the optimal conditions, the aptasensor reached an impressively low detection limit 95.4 pM in colorimetric mode and 83.6 fM in electrochemical mode. We believe this dual-mode approach holds promise for veterinary drug residue detection.

Keywords: Colorimetric/electrochemical; M-TDN; Malachite green; P-CeO(2)NR@Mxene; Signal amplification.