Objective: To investigate the relationship between clinical indicators of CRAB symptoms and antioxidant enzyme activity in patients with multiple myeloma (MM).
Methods: The activity of catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD) in the bone marrow supernatants of 44 patients with MM and 12 patients with non-malignant hematological diseases was detected by colorimetric assay, and then the differences in the activity of antioxidant enzymes between the two groups were compared. Furthermore, the relationship between the activity of antioxidant enzymes in the MM group and the levels of serum calcium, serum creatinine (Scr), hemoglobin (Hb), alkaline phosphatase (ALP) as well as bone lesions were analyzed.
Results: The antioxidant enzyme activity was lower in MM patients compared with the control group (P < 0.05). When the concentrations of serum calcium and ALP were higher than the normal levels, Hb was lower than 85 g/L, and there were multiple bone lesions, the activity of CAT, SOD and GPX was significantly declined (P < 0.05); When the concentration of Scr≥177 μmol/L, the activity of GPX was significantly declined (P < 0.05). Regression analyses showed that CAT, SOD and GPX were negatively correlated with serum calcium (r =-0.538, r =-0.456, r =-0.431), Scr (r =-0.342, r =-0.384, r =-0.463), and ALP (r =-0.551, r =-0.572, r =-0.482).
Conclusion: The activity of antioxidant enzymes, including CAT, SOD and GPX, were decreased in patients with MM and they were negatively correlated with some clinical indicators of CRAB symptoms (such as serum calcium, Scr, and ALP), which suggests that promoting the activity of antioxidant enzymes may be beneficial to treat the CRAB symptoms of the patients with MM.
题目: 多发性骨髓瘤CRAB症状与抗氧化酶活性的相关性分析.
目的: 探讨多发性骨髓瘤（MM）患者CRAB症状的常见临床指标与抗氧化酶活性之间的联系。.
方法: 比色法检测44名骨髓瘤患者及12名非恶性血液病患者骨髓上清中的过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）和超氧化物歧化酶（SOD）的活性，分析两组抗氧化酶的活性差异以及骨髓瘤组中抗氧化酶的活性与血钙、血肌酐（Scr）、血红蛋白（Hb）、碱性磷酸酶（ALP）及骨破坏区域数量的关系。.
结果: 与对照组相比，骨髓瘤患者的抗氧化酶活性更低（P <0.05）。当血钙、ALP的浓度高于正常水平，Hb低于85 g/L及存在多个骨破坏区域时，抗氧化酶CAT、SOD和GPX的活性明显降低（P <0.05）；当Scr高于正常水平时，GPX活性明显降低（P <0.05）。回归分析结果显示，CAT、SOD、GPX与血钙（r =-0.538，r =-0.456，r =-0.431）、Scr（r =-0.342，r =-0.384，r =-0.463）和ALP（r =-0.551，r =-0.572，r =-0.482）均呈负性相关。.
结论: 抗氧化酶CAT、SOD和GPX的活性在多发性骨髓瘤患者中下降，并且与CRAB症状的常见临床指标（血钙、Scr、ALP）呈负性相关，或许可以通过提高骨髓瘤患者体内的抗氧化酶活性来治疗CRAB症状。.
Keywords: CRAB symptoms; catalase; glutathione peroxidase; multiple myeloma; superoxide dismutase.