CRISPR screens in mechanism and target discovery for AML

Tian Lin; Dan Liu; Zhangchun Guan; Xuan Zhao; Sijin Li; Xu Wang; Rui Hou; Junnian Zheng; Jiang Cao; Ming Shi

doi:10.1016/j.heliyon.2024.e29382

CRISPR screens in mechanism and target discovery for AML

Heliyon. 2024 Apr 9;10(8):e29382. doi: 10.1016/j.heliyon.2024.e29382. eCollection 2024 Apr 30.

Authors

Tian Lin^{1

2

3}, Dan Liu^{1

2

3}, Zhangchun Guan^{1

2

3}, Xuan Zhao^{1

2

3}, Sijin Li^{1

2

3}, Xu Wang^{1

2

3}, Rui Hou^{1

2

4}, Junnian Zheng^{2

3}, Jiang Cao⁵, Ming Shi^{1

2

3}

Affiliations

¹ Cancer Institute, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu, 221004, China.
² Center of Clinical Oncology, The Affiliated Hospital of Xuzhou Medical University, 99 Huaihai Road, Xuzhou, Jiangsu, 221002, China.
³ Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu, 221004, China.
⁴ College of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
⁵ Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, 99 Huaihai Road, Xuzhou, Jiangsu, 221002, China.

Abstract

CRISPR-based screens have discovered novel functional genes involving in diverse tumor biology and elucidated the mechanisms of the cancer pathological states. Recently, with its randomness and unbiasedness, CRISPR screens have been used to discover effector genes with previously unknown roles for AML. Those novel targets are related to AML survival resembled cellular pathways mediating epigenetics, synthetic lethality, transcriptional regulation, mitochondrial and energy metabolism. Other genes that are crucial for pharmaceutical targeting and drug resistance have also been identified. With the rapid development of novel strategies, such as barcodes and multiplexed mosaic CRISPR perturbation, more potential therapeutic targets and mechanism in AML will be discovered. In this review, we present an overview of recent progresses in the development of CRISPR-based screens for the mechanism and target identification in AML and discuss the challenges and possible solutions in this rapidly growing field.

Keywords: AML; CRISPR screens; Mechanism; Therapeutic targets.

Publication types

Review