Synergistic antimicrobial action of nanocellulose, nanoselenium, and nanocomposite against pathogenic microorganisms

Mostafa M El-Sheekh; Wesam E Yousuf; Tarek M Mohamed; El-Refaie Kenawy

doi:10.1016/j.ijbiomac.2024.131737

Synergistic antimicrobial action of nanocellulose, nanoselenium, and nanocomposite against pathogenic microorganisms

Int J Biol Macromol. 2024 Apr 22;268(Pt 2):131737. doi: 10.1016/j.ijbiomac.2024.131737. Online ahead of print.

Authors

Mostafa M El-Sheekh¹, Wesam E Yousuf², Tarek M Mohamed², El-Refaie Kenawy³

Affiliations

¹ Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt. Electronic address: mostafaelsheikh@science.tanta.edu.eg.
² Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
³ Polymer Research Group Chemistry Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.

PMID: 38657940
DOI: 10.1016/j.ijbiomac.2024.131737

Abstract

Recently, there has been a surge in curiosity regarding the application of biopolymer-derived nanomaterials, primarily attributable to their extensive array of potential applications. In this study, nanocellulose was extracted from algae, biomolecule substances synthesized selenium nanoparticles, and a simple nanocomposite of nanocellulose and nanoselenium was elaborated using nanocellulose as a reducing agent under hydrothermal conditions. These nanocomposite materials have markedly improved properties at low concentrations. Our obtained polymers were characterized using techniques including Fourier-transform infrared spectroscopy, X-ray powder diffraction, Thermo gravimetric analysis (TGA), Scanning electron microscopic (SEM), Energy Dispersive X-ray analysis (EDX), Transmission electron microscopic (TEM), Zeta Potential and Dynamic Light Scattering (DLS). The size of nanocellulose, nanoselenium, and nanocomposite ranged from 35 to 85 nm. Antimicrobial investigation of the prepared nanopolymers was tested against Gram-negative bacteria such as Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538, Gram-positive bacteria such as Escherichia coli ATCC8739 and Pseudomonas aeruginosa ATCC 90274 and fungi such as Candida albicans ATCC 10221 besides Aspergillus fumigatus. In antibacterial action tests, nanoselenium showed significant efficacy against Bacillus subtilis with a 12 mm zone of inhibition, while the nanocomposite eclipsed all microorganisms. Nanocellulose and the nanocomposite were potent against Staphylococcus aureus (14 mm and 16 mm zones of inhibition, respectively). The nanocomposite showed potential against Escherichia coli and Pseudomonas aeruginosa (17 mm and 15 mm zones of inhibition, respectively). All polymers effectively inhibited Candida albicans growth (18 mm for the nanocomposite). The minimum inhibitory concentrations (MIC) for three polymers have also been established. While nanocellulose displayed a MIC of 62.5 μg/ml in contradiction to Staphylococcus aureus, nanoselenium demonstrated a significant MIC of 3.95 μg/ml against Bacillus subtilis. These findings highlight the potential of the nanocomposite (nanocellulose-nanoselenium) as a broad-spectrum antimicrobial polymer.

Keywords: And MIC test; Antimicrobial activity; Nanocellulose; Nanocomposite; Nanoselenium.