Analysis of Key Factors for Evaluating Mucosal Adhesion Using Swine Buccal Tissue

Aylla Mesquita Pestana; Giovana Maria Fioramonti Calixto; Arthur Antunes Costa Bezerra; Lígia Nunes de Morais Ribeiro; Aline Carvalho da Costa; Ângela Maria Moraes; Michelle Franz-Montan

doi:10.1016/j.xphs.2024.04.018

Analysis of Key Factors for Evaluating Mucosal Adhesion Using Swine Buccal Tissue

J Pharm Sci. 2024 Apr 22:S0022-3549(24)00145-X. doi: 10.1016/j.xphs.2024.04.018. Online ahead of print.

Authors

Aylla Mesquita Pestana¹, Giovana Maria Fioramonti Calixto¹, Arthur Antunes Costa Bezerra¹, Lígia Nunes de Morais Ribeiro², Aline Carvalho da Costa³, Ângela Maria Moraes⁴, Michelle Franz-Montan⁵

Affiliations

¹ Department of Biosciences, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo 13414-903, Brazil.
² Bionanotechnology Laboratory Professor Luiz Ricardo Goulart Filho, Institute of Biotechnology, Federal University of Uberlândia, Minas Gerais 38400-902, Brazil.
³ Department of Process and Product Development, School of Chemical Engineering, University of Campinas, Campinas, São Paulo, 13083-970, Brazil.
⁴ Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas, São Paulo, 13083-970, Brazil.
⁵ Department of Biosciences, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo 13414-903, Brazil. Electronic address: mfranz@unicamp.br.

PMID: 38657756
DOI: 10.1016/j.xphs.2024.04.018

Abstract

The assessment of the mucoadhesive properties peak mucoadhesive force (F_max) and work of mucoadhesion (W_muc) with texture analyzers is a common in vitro method for analyzing formulation capabilities. Challenges arise in selecting and standardizing experimental conditions due to various variables influencing mucoadhesion. This complexity hampers direct product performance comparisons. In our study, we explored factors (contact force and time, probe speed and mucin in artificial saliva) impacting a model formulation's mucoadhesive capacity. Using Omcilon-A®Orabase on porcine buccal mucosa, we systematically varied experimental conditions, employing a statistical approach (Central Composite Design - CCD). Three variables (contact force, contact time, probe speed) and their interactions were assessed for their impact on F_max and W_muc. Results showed that contact time and force positively affected F_max, while only contact time influenced W_muc. In the mucin artificial saliva test, a force of 0.5 N, time of 600 s, and speed of 1 mm/s yielded optimal F_max (0.587 N) and W_muc (0.468 N.s). These conditions serve as a reference for comparing mucoadhesive properties of formulations for topical oral use.

Keywords: Buccal delivery; Composite design; Factorial design; Mucoadhesive; Mucosal delivery; Mucosal drug delivery; Texture analyzer; Topical formulation.