Two-Dimensional Gel Electrophoresis for Protein Separation of Plant Samples

Gorji Marzban; Donatella Tesei

doi:10.1007/978-1-0716-3778-4_19

Two-Dimensional Gel Electrophoresis for Protein Separation of Plant Samples

Methods Mol Biol. 2024:2787:281-291. doi: 10.1007/978-1-0716-3778-4_19.

Authors

Gorji Marzban¹, Donatella Tesei^{2

3}

Affiliations

¹ Institute for Bioprocess Science and Engineering (IBSE), Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria. gorji.marzban@boku.ac.at.
² Institute of Microbiology and Microbial Biotechnology (IMMB), Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
³ State Key Laboratory of Lunar and Planetary Science, Macau University of Science and Technology, Macau, China.

PMID: 38656497
DOI: 10.1007/978-1-0716-3778-4_19

Abstract

This chapter provides a description of the procedure for two-dimensional electrophoresis that can be performed for any given gel size and isoelectric focusing range. This will enable the operator to recognize critical steps and gain sufficient information to generate 2D images suitable for computer-assisted analysis of 2D-gel, as well as mass spectrometry analysis for protein identification and characterization.

Keywords: Gel documentation; Immobilized pH-gradient; Isoelectric focusing; MS-compatible silver staining; SDS-PAGE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Gel, Two-Dimensional* / methods
Isoelectric Focusing* / methods
Mass Spectrometry / methods
Plant Proteins* / analysis
Plant Proteins* / isolation & purification
Plants / chemistry
Proteomics / methods

Substances

Plant Proteins