Characterization of the complete chloroplast genome sequence of Agave durangensis (Asparagales: Asparagaceae: Agavoideae)

Ana Carolina González-Trillo; Miguel Ángel Reyes López; Norma Almaraz-Abarca; Yolanda Herrera-Arrieta; Marcela Verónica Gutiérrez-Velázquez; Marcelo Barraza Salas; Hugo Manuel Monreal-García; Rene Torres-Ricario

doi:10.1080/23802359.2024.2338546

Characterization of the complete chloroplast genome sequence of Agave durangensis (Asparagales: Asparagaceae: Agavoideae)

Mitochondrial DNA B Resour. 2024 Apr 22;9(4):536-540. doi: 10.1080/23802359.2024.2338546. eCollection 2024.

Authors

Ana Carolina González-Trillo¹, Miguel Ángel Reyes López², Norma Almaraz-Abarca¹, Yolanda Herrera-Arrieta¹, Marcela Verónica Gutiérrez-Velázquez¹, Marcelo Barraza Salas³, Hugo Manuel Monreal-García^{1

2

3}, Rene Torres-Ricario¹

Affiliations

¹ Instituto Politécnico Nacional CIIDIR Unidad Durango, Durango, Durango, Mexico.
² Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, Mexico.
³ Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Durango, Durango, Mexico.

Abstract

Agave durangensis commonly known as agave cenizo, is an endemic Agave species in Mexico used for mescal production, yet its taxonomic delimitation is still controversial. This study aimed to enhance taxonomic clarity by characterizing its chloroplast genome. Chloroplast DNA was isolated from 2-year-old A. durangensis leaves. The complete chloroplast genome size was 156,441 bp, comprising a large single-copy region (LSC), a pair of inverted repeat regions (IR), and a small single-copy region (SSC). Annotation revealed 87 protein-coding genes, 38 tRNAs, and 8 rRNAs, with notable gene inversions. Phylogenetic analysis suggests, A. durangensis forms a separate lineage within the Agave genus.

Keywords: Agave durangensis; Chloroplast genome; phylogenetic analysis.

Grants and funding

This research was supported by the National Polytechnic Institute (Multidisciplinary and Transdisciplinary Projects of Scientific Research and Technological Development 20230049 and SIP 20240599) and The National Council of Science and Technology (CONACyT), Mexico, with the grant (428299) to one of the authors.