Proteomic biomarkers in seminal plasma as predictors of reproductive potential in azoospermic men

Front Endocrinol (Lausanne). 2024 Apr 9:15:1327800. doi: 10.3389/fendo.2024.1327800. eCollection 2024.

Abstract

Introduction: Azoospermia, characterized by an absence of sperm in the ejaculate, represents the most severe form of male infertility. While surgical sperm retrieval in obstructive azoospermia (OA) is successful in the majority of cases, patients with non-obstructive azoospermia (NOA) show retrieval rates of only about 50% and thus frequently have unnecessary surgery. Surgical intervention could be avoided if patients without preserved spermatogenesis are identified preoperatively. This prospective study aimed to discover biomarkers in seminal plasma that could be employed for a non-invasive differential diagnosis of OA/NOA in order to rationalize surgery recommendations and improve success rates.

Methods: All patients signed written informed consent, underwent comprehensive andrological evaluation, received human genetics to exclude relevant pathologies, and patients with azoospermia underwent surgical sperm retrieval. Using label-free LC-MS/MS, we compared the proteomes of seminal plasma samples from fertile men (healthy controls (HC), n=8) and infertile men diagnosed with 1) OA (n=7), 2) NOA with successful sperm retrieval (mixed testicular atrophy (MTA), n=8), and 3) NOA without sperm retrieval (Sertoli cell-only phenotype (SCO), n=7). Relative abundance changes of two candidate markers of sperm retrieval, HSPA2 and LDHC, were confirmed by Western Blot.

Results: We found the protein expression levels of 42 proteins to be significantly down-regulated (p ≤ 0.05) in seminal plasma from SCO NOA patients relative to HC whereas only one protein was down-regulated in seminal plasma from MTA patients. Analysis of tissue and cell expression suggested that the testis-specific proteins LDHC, PGK2, DPEP3, and germ-cell enriched heat-shock proteins HSPA2 and HSPA4L are promising biomarkers of spermatogenic function. Western blotting revealed a significantly lower abundance of LDHC and HSPA2 in the seminal plasma of men with NOA (SCO and MTA) compared to controls.

Discussion: The results indicate that certain testis-specific proteins when measured in seminal plasma, could serve as indicators of the presence of sperm in the testis and predict the success of sperm retrieval. Used in conjunction with conventional clinical assessments, these proteomic biomarkers may assist in the non-invasive diagnosis of idiopathic male infertility.

Keywords: azoospermia; biomarker; diagnosis; proteomics; seminal plasma; testicular failure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Azoospermia* / diagnosis
  • Azoospermia* / metabolism
  • Biomarkers* / analysis
  • Biomarkers* / blood
  • Biomarkers* / metabolism
  • Case-Control Studies
  • Humans
  • Male
  • Prospective Studies
  • Proteomics* / methods
  • Semen* / chemistry
  • Semen* / metabolism
  • Sperm Retrieval
  • Spermatogenesis / physiology

Substances

  • Biomarkers

Supplementary concepts

  • Azoospermia, Nonobstructive

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by NHMRC Project Grant #10099002, the Victorian State Government Operational Infrastructure Support Program, and the Deutsche Forschungsgemeinschaft (DFG) within the framework of the International Research Training Group “Molecular pathogenesis of male reproductive disorders (DFG GRK/IRTG 1871/P4)”, a collaboration program of Justus-Liebig-University (Giessen, Germany) and Monash University (Clayton, Australia). This study was part of the doctoral thesis of R. Sgaier (62).