Methodological advances enabled by the construction of a synthetic yeast genome

Daniel Schindler; Roy S K Walker; Yizhi Cai

doi:10.1016/j.crmeth.2024.100761

Methodological advances enabled by the construction of a synthetic yeast genome

Cell Rep Methods. 2024 Apr 22;4(4):100761. doi: 10.1016/j.crmeth.2024.100761.

Authors

Daniel Schindler¹, Roy S K Walker², Yizhi Cai³

Affiliations

¹ Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany; Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, 35032 Marburg, Germany. Electronic address: daniel.schindler@mpi-marburg.mpg.de.
² School of Natural Sciences and ARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW 2109, Australia.
³ Manchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK. Electronic address: yizhi.cai@manchester.ac.uk.

Abstract

The international Synthetic Yeast Project (Sc2.0) aims to construct the first synthetic designer eukaryote genome. Over the past few years, the Sc2.0 consortium has achieved several significant milestones by synthesizing and characterizing all 16 nuclear chromosomes of the yeast Saccharomyces cerevisiae, as well as a 17^thde novo neochromosome containing all nuclear tRNA genes. In this commentary, we discuss the recent technological advances achieved in this project and provide a perspective on how they will impact the emerging field of synthetic genomics in the future.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genetic Engineering / methods
Genome, Fungal* / genetics
Genomics / methods
Saccharomyces cerevisiae* / genetics
Synthetic Biology / methods