Two previously undescribed coumarins (1 and 2) were isolated from the root of Hansenia weberbaueriana which have been used to cure inflammatory diseases over thousands of years by Chinese. The structures of new findings were confirmed by comprehensive analyses of spectral evidences in HRESIMS, 1D and 2D NMR combined with chemical calculations. Compounds 1 and 2 exhibited potential anti-inflammatory properties by reducing the mRNA expression levels of TNF-α, IL-6 and IL-1β in lipopolysaccharide (LPS)-induced RAW264.7 macrophages at a concentration of 15 μM.
Keywords: Coumarin; Hansenia weberbaueriana; anti-inflammatory activity.