Measurements of Oxygen Evolution in Photosynthesis

Dmitry Shevela; Wolfgang P Schröder; Johannes Messinger

doi:10.1007/978-1-0716-3790-6_8

Measurements of Oxygen Evolution in Photosynthesis

Methods Mol Biol. 2024:2790:133-148. doi: 10.1007/978-1-0716-3790-6_8.

Authors

Dmitry Shevela¹, Wolfgang P Schröder², Johannes Messinger^{3

4}

Affiliations

¹ Department of Chemistry, Umeå University, Umeå, Sweden. dmitry.shevela@umu.se.
² Department of Chemistry, Umeå University, Umeå, Sweden.
³ Department of Chemistry, Umeå University, Umeå, Sweden. johannes.messinger@kemi.uu.se.
⁴ Department of Chemistry - Ångström, Uppsala University, Uppsala, Sweden. johannes.messinger@kemi.uu.se.

PMID: 38649570
DOI: 10.1007/978-1-0716-3790-6_8

Abstract

This chapter compares two different techniques for monitoring photosynthetic O₂ production; the wide-spread Clark-type O₂ electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O₂ evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.

Keywords: Clark-type electrode; Membrane-inlet mass spectrometry; O2 evolution; Oxygenic photosynthesis; Photosynthetic water oxidation; Photosynthetic water splitting; Photosystem II.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorophyll / metabolism
Electrodes
Mass Spectrometry* / methods
Oxygen* / metabolism
Photosynthesis*
Photosystem II Protein Complex* / metabolism

Substances

Oxygen
Photosystem II Protein Complex
Chlorophyll