Investigations of membrane protein interactions in cells using fluorescence microscopy

Mahmoud Abouelkheir; Tanaya Roy; Mateusz A Krzyscik; Ece Özdemir; Kalina Hristova

doi:10.1016/j.sbi.2024.102816

Investigations of membrane protein interactions in cells using fluorescence microscopy

Curr Opin Struct Biol. 2024 Apr 21:86:102816. doi: 10.1016/j.sbi.2024.102816. Online ahead of print.

Authors

Mahmoud Abouelkheir¹, Tanaya Roy², Mateusz A Krzyscik², Ece Özdemir², Kalina Hristova³

Affiliations

¹ Department of Materials Science and Engineering and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore MD 21218, USA; Chemistry-Biology Interface Program, Johns Hopkins University, Baltimore MD 21218, USA.
² Department of Materials Science and Engineering and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore MD 21218, USA.
³ Department of Materials Science and Engineering and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore MD 21218, USA; Chemistry-Biology Interface Program, Johns Hopkins University, Baltimore MD 21218, USA. Electronic address: kh@jhu.edu.

PMID: 38648680
DOI: 10.1016/j.sbi.2024.102816

Abstract

The interactions between proteins in membranes govern many cellular functions. Our ability to probe for such interactions has greatly evolved in recent years due to the introduction of new fluorescence techniques. As a result, we currently have a choice of methods that can be used to assess the spatial distribution of a membrane protein, its association state, and the thermodynamic stability of the oligomers in the native milieu. These biophysical measurements have revealed new insights into important biological processes in cellular membranes.

Keywords: Fluorescence; Interactions; Membrane proteins; Receptors.

Publication types

Review