A multi-component reaction for covalent immobilization of lipases on amine-functionalized magnetic nanoparticles: production of biodiesel from waste cooking oil

Yalda Amini; Mansour Shahedi; Zohreh Habibi; Maryam Yousefi; Maryam Ashjari; Mehdi Mohammadi

doi:10.1186/s40643-022-00552-0

A multi-component reaction for covalent immobilization of lipases on amine-functionalized magnetic nanoparticles: production of biodiesel from waste cooking oil

Bioresour Bioprocess. 2022 May 30;9(1):60. doi: 10.1186/s40643-022-00552-0.

Authors

Yalda Amini¹, Mansour Shahedi¹, Zohreh Habibi², Maryam Yousefi³, Maryam Ashjari^{1

4}, Mehdi Mohammadi⁵

Affiliations

¹ Department of Organic Chemistry and Oil, Faculty of Chemistry, Shahid Beheshti University, Tehran, Iran.
² Department of Organic Chemistry and Oil, Faculty of Chemistry, Shahid Beheshti University, Tehran, Iran. Z_habibi@sbu.ac.ir.
³ Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
⁴ Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
⁵ Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. m.mohammadi@nigeb.ac.ir.

Abstract

A new approach was used for the immobilization of Thermomyces lanuginosus lipase (TLL), Candida antarctica lipase B (CALB), and Rhizomucor miehei lipase (RML) on amine-functionalized magnetic nanoparticles (Fe₃O₄@SiO₂-NH₂) via a multi-component reaction route (using cyclohexyl isocyanide). The used method offered a single-step and very fast process for covalent attachment of the lipases under extremely mild reaction conditions (25 °C, water, and pH 7.0). Rapid and simple immobilization of 20 mg of RML, TLL, and CALB on 1 g of the support produced 100%, 98.5%, and 99.2% immobilization yields, respectively, after 2 h of incubation. The immobilized derivatives were then used for biodiesel production from waste cooking oil. Response surface methodology (RSM) in combination with central composite rotatable design (CCRD) was employed to evaluate and optimize the biodiesel production. The effect of some parameters such as catalyst amount, reaction temperature, methanol concentration, water content for TLL or water-adsorbent for RML and CALB, and ratio of t-butanol (wt%) were investigated on the fatty acid methyl esters (FAME) yield.

Keywords: Biodiesel; Covalent immobilization; Lipase; Magnetic nanoparticles; Multi-component reaction.