GmCYB5-4 inhibit SMV proliferation by targeting P3 protein

Daiqiao Song; Kai Huang; Shuxin Li; Jia Jiang; Longgang Zhao; Hexiang Luan

doi:10.1016/j.virol.2024.110069

GmCYB5-4 inhibit SMV proliferation by targeting P3 protein

Virology. 2024 Apr 10:595:110069. doi: 10.1016/j.virol.2024.110069. Online ahead of print.

Authors

Daiqiao Song¹, Kai Huang¹, Shuxin Li¹, Jia Jiang², Longgang Zhao³, Hexiang Luan⁴

Affiliations

¹ Institute of Plant Genetic Engineering, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, Shandong, China.
² Hospital of Qingdao Agricultural University, Qingdao, 266109, China.
³ College of Grassland Science, Qingdao Agricultural University, Qingdao, 266109, China; High-efficiency Agricultural Technology Industry Research Institute of Saline and alkaline Land of Dongying Qingdao Agricultural University, China. Electronic address: zhaolonggang@qau.edu.cn.
⁴ Institute of Plant Genetic Engineering, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, Shandong, China; High-efficiency Agricultural Technology Industry Research Institute of Saline and alkaline Land of Dongying Qingdao Agricultural University, China. Electronic address: hexiangl@qau.edu.cn.

PMID: 38640788
DOI: 10.1016/j.virol.2024.110069

Abstract

Soybean mosaic virus (SMV) is a potyvirus found worldwide in soybean (Glycine max). GmCYB5-4 is a strong candidate interactor of P3. In this study, we comprehensively analyzed the GmCYB5 family in soybeans, including its distribution on chromosomes, promoter analysis, conserved motifs, phylogenetic analysis, and expression patterns. We cloned the full-length GmCYB5-4 and examined its interaction with P3 in yeast, which was later confirmed using bimolecular fluorescence complementation (BiFc). We silenced GmCYB5-4 using a bean pottle mosaic viris (BPMV) based system to generate Sil_CYB5-4 tissues, which surprisingly knocked down four isoforms of GmCYB5s for functional characterization. Sil_CYB5-4 plants were challenged with the SC3 strain to determine its involvement in SMV infection. Silencing GmCYB5-4 increased SMV accumulation, indicating that GmCYB5-4 inhibited SMV proliferation. However, further experiments are needed to elucidate the mechanism underlying the involvement of GmCYB5-4 in SMV infection.

Keywords: Cytochrome b5; P3 protein; Soybean mosaic virus; Virus-induced gene silencing.