Sensitive colorimetric and fluorescence dual-mode detection of thiophanate-methyl based on spherical Fe3O4/GONRs composite nanozyme

Shengmei Tai; Hui Cao; Yingkang Cui; Chifang Peng; Jianguo Xu; Zhouping Wang

doi:10.1016/j.foodchem.2024.139258

Sensitive colorimetric and fluorescence dual-mode detection of thiophanate-methyl based on spherical Fe₃O₄/GONRs composite nanozyme

Food Chem. 2024 Apr 4:450:139258. doi: 10.1016/j.foodchem.2024.139258. Online ahead of print.

Authors

Shengmei Tai¹, Hui Cao², Yingkang Cui³, Chifang Peng⁴, Jianguo Xu⁵, Zhouping Wang⁶

Affiliations

¹ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
³ School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
⁴ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China; International Joint Laboratory On Food Safety, Jiangnan University, Wuxi 214122, China. Electronic address: pcf@jiangnan.edu.
⁵ Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China.
⁶ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China; International Joint Laboratory On Food Safety, Jiangnan University, Wuxi 214122, China.

PMID: 38626710
DOI: 10.1016/j.foodchem.2024.139258

Abstract

Pesticide detection based on nanozyme is largely limited in terms of the variety of pesticides. Herein, a spherical and well-dispersed Fe₃O₄/graphene oxide nanoribbons (Fe₃O₄/GONRs) composite nanozyme was applied to firstly develop an enzyme-free and sensitive colorimetric and fluorescence dual-mode detection of thiophanate-methyl (TM). The synthesized Fe₃O₄/GONRs possess excellent dual enzyme-like activities (peroxidase and catalase) and can catalyze H₂O₂ to oxidize 3,3',5,5'-tetramethylbenzidine (TMB) into oxidized TMB (oxTMB). We found that Fe₃O₄/GONRs can adsorb TM through the synergistic effect of multiple forces, thereby inhibiting the catalytic activities of nanozyme. This inhibition can modulate the transformation of TMB to oxTMB, producing dual responses of absorbance decrease (oxTMB) and fluorescence enhancement (TMB). The limits of detection (LODs) of TM were 28.1 ng/mL (colorimetric) and 8.81 ng/mL (fluorescence), respectively. Moreover, the developed method with the recoveries of 94.8-100.8% also exhibited a good potential application in the detection of pesticides residues in water and food samples.

Keywords: Colorimetric; Fluorescence; Graphene oxide nanoribbons; Magnetic nanoparticle; Nanozyme; Thiophanate-methyl.