Phosphate-solubilizing bacteria improve the antioxidant enzyme activity of Potamogeton crispus L. and enhance the remediation effect on Cd-contaminated sediment

Yuxin Cheng; Junjun Yuan; Gongting Wang; Zhenzhen Hu; Wenqing Luo; Xin Zhao; Yali Guo; Xiaonan Ji; Wei Hu; Min Li

doi:10.1016/j.jhazmat.2024.134305

Phosphate-solubilizing bacteria improve the antioxidant enzyme activity of Potamogeton crispus L. and enhance the remediation effect on Cd-contaminated sediment

J Hazard Mater. 2024 May 15:470:134305. doi: 10.1016/j.jhazmat.2024.134305. Epub 2024 Apr 12.

Authors

Yuxin Cheng¹, Junjun Yuan¹, Gongting Wang¹, Zhenzhen Hu¹, Wenqing Luo¹, Xin Zhao¹, Yali Guo², Xiaonan Ji², Wei Hu², Min Li³

Affiliations

¹ Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China.
² Shanghai Investigation, Design & Research Institute Co., Ltd., Shanghai 200335, China; YANGTZE Eco-Environment Engineering Research Center (Shanghai), China Three Gorges Corporation, Shanghai 200335, China.
³ Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China. Electronic address: minli@bjfu.edu.cn.

PMID: 38626677
DOI: 10.1016/j.jhazmat.2024.134305

Abstract

Phosphorus-solubilizing bacteria (PSB) assisted phytoremediation of cadmium (Cd) pollution is an effective method, but the mechanism of PSB-enhanced in-situ remediation of Cd contaminated sediment by submerged plants is still rare. In this study, PSB (Leclercia adecarboxylata L1-5) was inoculated in the rhizosphere of Potamogeton crispus L. (P. crispus) to explore the effect of PSB on phytoremediation. The results showed that the inoculation of PSB effectively improved the Cd extraction by P. crispus under different Cd pollution and the Cd content in the aboveground and underground parts of P. crispus all increased. The μ-XRF images showed that most of the Cd was enriched in the roots of P. crispus. PSB especially showed positive effects on root development and chlorophyll synthesis. The root length of P. crispus increased by 51.7 %, 80.5 % and 74.2 % under different Cd pollution, and the Ca/Cb increased by 38.9 %, 15.2 % and 8.6 %, respectively. Furthermore, PSB enhanced the tolerance of P. crispus to Cd. The contents of soluble protein, MDA and H₂O₂ in 5 mg·kg^-1 and 7 mg·kg^-1 Cd content groups were decreased and the activities of antioxidant enzymes were increased after adding PSB. The results showed that the application of PSB was beneficial to the in-situ remediation of submerged plants.

Keywords: Cadmium; Phosphorus-solubilizing bacteria; Phytoremediation; Potamogeton crispus L.; Sediment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / metabolism
Bacteria / metabolism
Biodegradation, Environmental*
Cadmium* / metabolism
Cadmium* / toxicity
Geologic Sediments* / microbiology
Phosphates* / metabolism
Plant Roots* / drug effects
Plant Roots* / growth & development
Plant Roots* / metabolism
Potamogetonaceae* / metabolism
Rhizosphere
Soil Pollutants* / metabolism

Substances

Cadmium
Soil Pollutants
Phosphates
Antioxidants