Plasma Membrane (PM) H+-ATPase Mediates Rhizosphere Acidification and Regulates Herbicide Imazethapyr Toxicity in Wheat

Chenyang Fan; Jun Li; Siyuan Dai; Xuan Xuan; Dongmei Xu; Yuezhong Wen

doi:10.1021/acs.jafc.4c00313

Plasma Membrane (PM) H⁺-ATPase Mediates Rhizosphere Acidification and Regulates Herbicide Imazethapyr Toxicity in Wheat

J Agric Food Chem. 2024 Apr 16. doi: 10.1021/acs.jafc.4c00313. Online ahead of print.

Authors

Chenyang Fan¹, Jun Li¹, Siyuan Dai¹, Xuan Xuan², Dongmei Xu², Yuezhong Wen¹

Affiliations

¹ MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
² Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.

PMID: 38623691
DOI: 10.1021/acs.jafc.4c00313

Abstract

The plasma membrane (PM) H⁺-ATPase is crucial for a plant defense system. However, there is currently no consensus on whether the PM H⁺-ATPase plays a role in alleviating the toxic effects of herbicides on nontarget plants. We found that under the herbicide imazethapyr (IM) exposure, PM H⁺-ATPase activity in wheat roots increased by approximately 69.53%, leading to rhizosphere acidification. When PM H⁺-ATPase activity is inhibited, the toxicity of IM significantly increases: When exposed to IM alone, the total Fe content of wheat roots decreased by 29.07%, the relative Fe²⁺ content increased by 27.75%, and the ROS content increased by 27.74%. When the PM H⁺-ATPase activity was inhibited, the corresponding data under IM exposure were 37.36%, 215%, and 57.68%, respectively. This work delves into the role of PM H⁺-ATPase in mediating the detoxification mechanism in plants exposed to herbicides, offering new insights into enhancing crop resistance against herbicides.

Keywords: PM H+-ATPase; herbicides; iron homeostasis; oxidative stress; rhizosphere acidification; wheat.