Simple enzyme based fluorimetric biosensor for urea in human biofluids

Karthika Lakshmi Servarayan; Ellairaja Sundaram; Kumaravel Velayutham; Manikka Kubendran Aravind; Manickam Sundarapandi; Balasubramaniem Ashokkumar; Vasantha Vairathevar Sivasamy

doi:10.1016/j.saa.2024.124271

Simple enzyme based fluorimetric biosensor for urea in human biofluids

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jul 5:315:124271. doi: 10.1016/j.saa.2024.124271. Epub 2024 Apr 8.

Authors

Karthika Lakshmi Servarayan¹, Ellairaja Sundaram², Kumaravel Velayutham³, Manikka Kubendran Aravind⁴, Manickam Sundarapandi⁵, Balasubramaniem Ashokkumar⁴, Vasantha Vairathevar Sivasamy⁶

Affiliations

¹ Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625 021, Tamilnadu, India.
² Department of Chemistry, Vivekananda College, Tiruvedakam West, Madurai 625 234, Tamilnadu, India.
³ Alpha Hospital and Research Center, Madurai 625 009, Tamilnadu, India.
⁴ Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, Tamilnadu, India.
⁵ Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 61005, South Korea.
⁶ Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625 021, Tamilnadu, India. Electronic address: vasantham999@yahoo.co.in.

PMID: 38613899
DOI: 10.1016/j.saa.2024.124271

Abstract

As an important biomarker for renal related diseases, detection of urea is playing a vital role in human biofluids on clinical diagnosis concern. In this work, a synthetic salicyaldehyde based imine fluorophore was synthesized using sonication method and conjugated with urease which was used as fluorescent biosensor for the detection of urea in serum samples. This enzyme based biosensor has shown a good selectivity and sensitivity towards urea with the linear range from 2 to 80 mM and the detection limit of 73 µM. The sensing response obtain is highly agreeing with existing analytical technique for urea detection which strongly recommends this biosensor for clinical application.

Keywords: Blood serum; Clinical analysis; Imine fluorophore; Urea; Urease.

MeSH terms

Biosensing Techniques* / methods
Enzymes, Immobilized / chemistry
Enzymes, Immobilized / metabolism
Fluorescent Dyes / chemistry
Fluorometry / methods
Humans
Limit of Detection
Spectrometry, Fluorescence / methods
Urea* / analysis
Urea* / blood
Urease* / chemistry
Urease* / metabolism

Substances

Urea
Urease
Fluorescent Dyes
Enzymes, Immobilized