Inhibition of Potato Fusarium Wilt by Bacillus subtilis ZWZ-19 and Trichoderma asperellum PT-29: A Comparative Analysis of Non-Targeted Metabolomics

Jianxiu Hao; Zhen Wang; Yuanzheng Zhao; Shujie Feng; Zining Cui; Yinqiang Zhang; Dong Wang; Hongyou Zhou

doi:10.3390/plants13070925

Inhibition of Potato Fusarium Wilt by Bacillus subtilis ZWZ-19 and Trichoderma asperellum PT-29: A Comparative Analysis of Non-Targeted Metabolomics

Plants (Basel). 2024 Mar 22;13(7):925. doi: 10.3390/plants13070925.

Authors

Jianxiu Hao¹, Zhen Wang^{1

2}, Yuanzheng Zhao³, Shujie Feng², Zining Cui², Yinqiang Zhang¹, Dong Wang¹, Hongyou Zhou¹

Affiliations

¹ Key Laboratory of Biopesticide Creation and Resource Utilization in Inner Mongolia Autonomous Region, College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010020, China.
² Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, China.
³ Institute of Plant Protection, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China.

Abstract

Potato Fusarium Wilt is a soil-borne fungal disease that can seriously harm potatoes throughout their growth period and occurs at different degrees in major potato-producing areas in China. To reduce the use of chemical agents and improve the effect of biocontrol agents, the inhibitory effects of the fermentation broth of Bacillus subtilis ZWZ-19 (B) and Trichoderma asperellum PT-29 (T) on Fusarium oxysporum were compared under single-culture and co-culture conditions. Furthermore, metabolomic analysis of the fermentation broths was conducted. The results showed that the inhibitory effect of the co-culture fermentation broth with an inoculation ratio of 1:1 (B1T1) was better than that of the separately cultured fermentation broths and had the best control effect in a potted experiment. Using LC-MS analysis, 134 metabolites were determined and classified into different types of amino acids. Furthermore, 10 metabolic pathways had the most significant variations, and 12 were related to amino acid metabolism in the KEGG analysis. A correlation analysis of the 79 differential metabolites generated through the comprehensive comparison between B, T, and B1T1 was conducted, and the results showed that highly abundant amino acids in B1T1 were correlated with amino acids in B, but not in T.

Keywords: Bacillus subtilis ZWZ-19; Trichoderma asperellum PT-29; fermentation broth; metabolomics analysis; potato Fusarium wilt.

Grants and funding

This research was funded by central guidance for local scientific and technological development funds, grant number 2023ZY0014; the Inner Mongolia Natural Science Foundation, grant number 2021BS03008; basic research business fees for universities directly under the Mongolian Autonomous Region, grant number BR22-11-14 and BR22-13-11; the Open Project of Guangdong Province Key Laboratory of Microbial Signals and Disease Control, grant number MSDC2023-04.