Resveratrol-Loaded Polydimethylsiloxane-Silica Hybrid Materials: Synthesis, Characterization, and Antitumoral Activity

Sofia Viegas; Diogo Marinheiro; Verónica Bastos; Ana L Daniel-da-Silva; Ricardo Vieira; Helena Oliveira; José Carlos Almeida; Bárbara J M L Ferreira

doi:10.3390/polym16070879

Resveratrol-Loaded Polydimethylsiloxane-Silica Hybrid Materials: Synthesis, Characterization, and Antitumoral Activity

Polymers (Basel). 2024 Mar 22;16(7):879. doi: 10.3390/polym16070879.

Authors

Sofia Viegas¹, Diogo Marinheiro¹, Verónica Bastos², Ana L Daniel-da-Silva¹, Ricardo Vieira¹, Helena Oliveira², José Carlos Almeida³, Bárbara J M L Ferreira¹

Affiliations

¹ Department of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal.
² Department of Biology, CESAM-Centre for Environmental and Marine Studies, University of Aveiro, 3810-193 Aveiro, Portugal.
³ Department of Materials and Ceramic Engineering, CICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal.

Abstract

In this work, hybrid materials within the polydimethylsiloxane-silica (PDMS-SiO₂) system, synthesized via the sol-gel method, were developed and characterized for their potential to incorporate and release the bioactive compound resveratrol (RES). RES was incorporated into the materials with a high loading efficiency (>75%) using the rotary evaporator technique. This incorporation induced the amorphization of RES, resulting in enhanced solubility and in vitro release when compared to the free polyphenolic compound. The release profiles displayed pH dependence, exhibiting notably faster release at pH 5.2 compared to pH 7.4. The gradual release of RES over time demonstrated an initial time lag of approximately 4 h, being well described by the Weibull model. In vitro cytotoxicity studies were conducted on human osteosarcoma cells (MG-63), revealing a concentration-dependent decrease in cell viability for RES-loaded samples (for concentrations >50 µg mL^-1).

Keywords: PDMS-SiO2 system; bone tissue; drug delivery; osteosarcoma; resveratrol; sol–gel hybrid materials.

Grants and funding

This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020, and LA/P/0006/2020, financed by national funds through the FCT/MCTES (PIDDAC). D.M. acknowledges Fundação para a Ciência e Tecnologia (FCT) for the PhD grant (2021.05673.BD). R.V. gratefully acknowledges FCT for the Junior Research Position (CEECIND/02127/2017). A.L.D.-d.-S. acknowledges FCT for funding (CEECIND/03075/2018/CP1559/CT0020; DOI: 10.54499/CEECIND/03075/2018/CP1559/CT0020). The position held by B.J.M.L.F. was funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in numbers 4, 5, and 6 of Article 23 of the Decreet-Law 57/2016, of 29 August, changed by Law 57/2017 of 19 July (DOI: 10.54499/DL57/2016/CP1482/CT0019). The authors acknowledge the financial support to CESAM by FCT/MCTES (UIDP/50017/2020, UIDB/50017/2020, and LA/P/0094/2020), through national funds. V.B. research contract is also acknowledged (CDL-CTTRI-54-SGRH/2022) and H.O. research contract under the FCT Scientific Employment Stimulus (CEECIND/04050/2017/CP1459/CT0023;DOI:10.54499/CEECIND/04050/2017/CP1459/CT0023)CEECIND/04050/2017).