In response to the issues of insufficient stability and accuracy in dry chemical detection using reflectance photometry, caused by the divergence and multiple internal reflections of the reflected light signal from the sample and the multilayer dry film test strip, a dry chemical reflectance photometry detection system based on an integrating sphere is designed. Firstly, an integrating sphere device is incorporated to reduce signal divergence and loss, ensuring even detection of the sample's reflected light signal and improving detection stability. Secondly, Light Tools optical simulation analysis is performed, and an integrating sphere detection model is established. Thirdly, the Williams-Clapper equation is employed to correct the error in reflectance density caused by multiple internal reflections, enhancing detection accuracy. Experimental validation demonstrates that the developed integrating sphere-based dry chemical reflectance photometry detection system improves the stability and accuracy of the detection system.
针对反射光度法的干化学检测中，因检测样品反射光信号的发散和多层膜干片试纸内部存在多重内反射，导致检测稳定性和准确性不足的问题，设计了一种基于积分球的干化学反射光度法检测系统。首先，增设积分球装置，可减少信号的发散与流失并均匀检测样品的反射光信号，提高检测的稳定性；其次，进行Light Tools光学仿真分析，并建立积分球检测模型；再次，基于Williams-Clapper方程对多重内反射导致的反射密度的误差进行修正以提高检测的准确性。实验验证：所开发的基于积分球的干化学反射光度法检测系统提高了检测系统的稳定性和准确性。.
Keywords: Williams-Clapper equation; integrating sphere; multiple internal reflections; reflectance photometry.