Ecology of Legionella pneumophila biofilms: The link between transcriptional activity and the biphasic cycle

Ana Barbosa; Nuno F Azevedo; Darla M Goeres; Laura Cerqueira

doi:10.1016/j.bioflm.2024.100196

Ecology of Legionella pneumophila biofilms: The link between transcriptional activity and the biphasic cycle

Biofilm. 2024 Mar 30:7:100196. doi: 10.1016/j.bioflm.2024.100196. eCollection 2024 Jun.

Authors

Ana Barbosa^{1

2}, Nuno F Azevedo^{1

2}, Darla M Goeres^{1

2

3}, Laura Cerqueira^{1

2}

Affiliations

¹ LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal.
² ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal.
³ The Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.

Abstract

There has been considerable discussion regarding the environmental life cycle of Legionella pneumophila and its virulence potential in natural and man-made water systems. On the other hand, the bacterium's morphogenetic mechanisms within host cells (amoeba and macrophages) have been well documented and are linked to its ability to transition from a non-virulent, replicative state to an infectious, transmissive state. Although the morphogenetic mechanisms associated with the formation and detachment of the L. pneumophila biofilm have also been described, the capacity of the bacteria to multiply extracellularly is not generally accepted. However, several studies have shown genetic pathways within the biofilm that resemble intracellular mechanisms. Understanding the functionality of L. pneumophila cells within a biofilm is fundamental for assessing the ecology and evaluating how the biofilm architecture influences L. pneumophila survival and persistence in water systems. This manuscript provides an overview of the biphasic cycle of L. pneumophila and its implications in associated intracellular mechanisms in amoeba. It also examines the molecular pathways and gene regulation involved in L. pneumophila biofilm formation and dissemination. A holistic analysis of the transcriptional activities in L. pneumophila biofilms is provided, combining the information of intracellular mechanisms in a comprehensive outline. Furthermore, this review discusses the techniques that can be used to study the morphogenetic states of the bacteria within biofilms, at the single cell and population levels.

Keywords: Biofilm ecology; Biofilm physiology; Cell functionality; Legionella pneumophila; Spatial organization.

Publication types

Review