Osteogenic differentiation of human mesenchymal stem cells on electroactive substrates

M N Tamaño-Machiavello; E O Carvalho; D Correia; L Cordón; S Lanceros-Méndez; A Sempere; R Sabater I Serra; J L Gómez Ribelles

doi:10.1016/j.heliyon.2024.e28880

Osteogenic differentiation of human mesenchymal stem cells on electroactive substrates

Heliyon. 2024 Mar 29;10(7):e28880. doi: 10.1016/j.heliyon.2024.e28880. eCollection 2024 Apr 15.

Authors

M N Tamaño-Machiavello¹, E O Carvalho², D Correia³, L Cordón^{4

5}, S Lanceros-Méndez^{2

6

7}, A Sempere^{4

5

8}, R Sabater I Serra^{1

9}, J L Gómez Ribelles^{1

9}

Affiliations

¹ Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 València, Spain.
² Centre of Physics, Universidade do Minho, 4710-057, Braga, Portugal.
³ Centre of Chemistry, University of Minho, 4710-057, Braga, Portugal.
⁴ Hematology Research Group, Instituto de Investigación Sanitaria La Fe, València, Spain.
⁵ Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Instituto Carlos III, Madrid, Spain.
⁶ BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940, Leioa, Spain.
⁷ IKERBASQUE, Basque Foundation for Science, 48009, Bilbao, Spain.
⁸ Hematology Department, Hospital Universitario y Politécnico La Fe, València, Spain.
⁹ CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain.

Abstract

This study investigates the effect of electroactivity and electrical charge distribution on the biological response of human bone marrow stem cells (hBMSCs) cultured in monolayer on flat poly(vinylidene fluoride), PVDF, substrates. Differences in cell behaviour, including proliferation, expression of multipotency markers CD90, CD105 and CD73, and expression of genes characteristic of different mesenchymal lineages, were observed both during expansion in basal medium before reaching confluence and in confluent cultures in osteogenic induction medium. The crystallisation of PVDF in the electrically neutral α-phase or in the electroactive phase β, both unpoled and poled, has been found to have an important influence on the biological response. In addition, the presence of a permanent positive or negative surface electrical charge distribution in phase β substrates has also shown a significant effect on cell behaviour.

Keywords: Electroactive cell substrates; Human bone marrow mesenchymal cells; Multipotency markers; poly(vinylidene fluoride).