LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy

Xiaodong Gu; Jingyuan Hou; Jiawei Rao; Ruiqiang Weng; Sudong Liu

doi:10.1016/j.heliyon.2024.e28882

LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy

Heliyon. 2024 Apr 3;10(7):e28882. doi: 10.1016/j.heliyon.2024.e28882. eCollection 2024 Apr 15.

Authors

Xiaodong Gu^{1

2

3}, Jingyuan Hou^{1

2

3}, Jiawei Rao⁴, Ruiqiang Weng^{1

2

3}, Sudong Liu^{1

2

3}

Affiliations

¹ Meizhou Clinical Institute, Shantou University Medical College, Meizhou, 514000, China.
² Research Experimental Center, Meizhou People's Hospital (Huangtang Hospital), Meizhou, 514031, China.
³ Guangdong Engineering Technology Research Center of Molecular Diagnostics for Cardiovascular Diseases, Meizhou, 514000, China.
⁴ Meizhou Clinical Medical School, Guangdong Medical University, Meizhou, 514000, China.

Abstract

Background: Monocyte-endothelial cell (EC) interactions are one of the earliest events in the development of atherosclerosis and play a crucial role in atherosclerotic plaque formation. Although attempts have been made to modulate this interaction, the underlying molecular signalling mechanisms remain unclear. This study aimed to investigate the role of long non-coding RNA MALAT1 in monocyte-EC interactions.

Methods: The expression of MALAT1, ICAM-1, VCAM-1, P-selectin, CCL2 and CXCL1 was evaluated in ApoE^-/- mouse aortic tissues and inflamed human umbilical vein endothelial cells (HUVECs). The regulatory impact of MALAT1 on cell adhesion molecules, monocyte-EC adhesion, and autophagy was assessed. The interactions between MALAT1 and microRNAs (miRNAs) were evaluated using dual-luciferase reporter and RNA pull-down assays.

Results: MALAT1 expression decreased in ApoE^-/- mouse aortic tissues and inflammatory HUVECs. MALAT1 overexpression suppressed the expression of ICAM-1, VCAM-1 and CXCL1, and reduced the migration and adhesion of monocytes to ECs. Inhibition of MALAT1 promoted cell adhesion molecule expression and monocyte-EC interactions. Mechanistically, MALAT1 binds directly to miR-30b-5p and decreases its effective expression by functioning as an endogenous sponge, thereby increasing the expression of autophagy-related gene 5 (ATG5) and stimulates endothelial autophagy.

Conclusions: Our findings suggest that MALAT1 suppresses monocyte-EC interactions by targeting miR-30b-5p and enhancing ATG5-mediated endothelial autophagy. These data imply that MALAT1 may play a protective role at the early stages of the atherosclerotic process.

Keywords: Atherosclerosis; Autophagy; Inflammation; Long non-coding RNA; Metastasis associated lung adenocarcinoma transcript 1; Monocyte-endothelial cell interaction.