On-site detection of infectious disease based on CaCO3-based magnetic micromotor integrated with graphene field effect transistor

Mikrochim Acta. 2024 Apr 11;191(5):257. doi: 10.1007/s00604-024-06345-w.

Abstract

A new detection platform based on CaCO3-based magnetic micromotor (CaCO3@Fe3O4) integrated with graphene field effect transistor (GFET) was construct and used for on-site SARS-CoV-2 coronavirus pathogen detection. The CaCO3@Fe3O4 micromotor, which was modified with anti-SARS-CoV-2 (labelled antibody, AntiE1), can self-moved in the solution containing hydrochloric acid (HCl) and effective to capture the SARS-CoV-2 coronavirus pathogens. After magnetic field separation, the capture micromotor was detected by GFET, exhibiting a good linear relationship within the range of 1 ag/mL to 100 ng/mL and low detection limit (0.39 ag/mL). Furthermore, the detection platform was also successfully applied to detection of SARS-CoV-2 coronavirus pathogens in soil solution, indicating the potential use in on-site application.

Keywords: GFET; Micromotor; On-site detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies
  • Communicable Diseases*
  • Graphite*
  • Humans
  • Magnetic Phenomena
  • SARS-CoV-2

Substances

  • Graphite
  • Antibodies