Proportions and Serogroups of Enterohemorrhagic Shiga Toxin-producing Escherichia coli in Feces of Fed and Cull Beef and Cull Dairy Cattle at Harvest

Joseph M Bosilevac; Tatum S Katz; Terrance M Arthur; Norasak Kalchayanand; Tommy L Wheeler

doi:10.1016/j.jfp.2024.100273

Proportions and Serogroups of Enterohemorrhagic Shiga Toxin-producing Escherichia coli in Feces of Fed and Cull Beef and Cull Dairy Cattle at Harvest

J Food Prot. 2024 Apr 8;87(6):100273. doi: 10.1016/j.jfp.2024.100273. Online ahead of print.

Authors

Joseph M Bosilevac¹, Tatum S Katz², Terrance M Arthur², Norasak Kalchayanand², Tommy L Wheeler²

Affiliations

¹ United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Meat Safety and Quality Research Unit, State Spur 18D, Clay Center, NE 68933, USA. Electronic address: mick.bosilevac@usda.gov.
² United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Meat Safety and Quality Research Unit, State Spur 18D, Clay Center, NE 68933, USA.

PMID: 38599382
DOI: 10.1016/j.jfp.2024.100273

Abstract

Cattle are considered a primary reservoir of Shiga toxin (stx)-producing Escherichia coli that cause enterohemorrhagic disease (EHEC), and contaminated beef products are one vehicle of transmission to humans. However, animals entering the beef harvest process originate from differing production systems: feedlots, dairies, and beef breeding herds. The objective of this study was to determine if fed cattle, cull dairy, and or cull beef cattle carry differing proportions and serogroups of EHEC at harvest. Feces were collected via rectoanal mucosal swabs (RAMSs) from 1,039 fed cattle, 1,058 cull dairy cattle, and 1,018 cull beef cattle at harvest plants in seven U.S. states (CA, GA, NE, PA, TX, WA, and WI). The proportion of the stx gene in feces of fed cattle (99.04%) was not significantly different (P > 0.05) than in the feces of cull dairy (92.06%) and cull beef (91.85%) cattle. When two additional factors predictive of EHEC (intimin and ecf1 genes) were considered, EHEC was significantly greater (P < 0.05) in fed cattle (77.29%) than in cull dairy (47.54%) and cull beef (38.51%) cattle. The presence of E. coli O157:H7 and five common non-O157 EHEC of serogroups O26, O103, O111, O121, and O145 was determined using molecular analysis for single nucleotide polymorphisms (SNPs) followed by culture isolation. SNP analysis identified 23.48%, 17.67%, and 10.81% and culture isolation confirmed 2.98%, 3.31%, and 3.00% of fed, cull dairy, and cull beef cattle feces to contain one of these EHEC, respectively. The most common serogroups confirmed by culture isolation were O157, O103, and O26. Potential EHEC of fourteen other serogroups were isolated as well, from 4.86%, 2.46%, and 2.01% of fed, cull dairy, and cull beef cattle feces, respectively; with the most common being serogroups O177, O74, O98, and O84. The identification of particular EHEC serogroups in different types of cattle at harvest may offer opportunities to improve food safety risk management.

Keywords: Cull beef cattle; Cull dairy cattle; Enterohemorrhagic E. coli; Feedlot cattle; Shiga toxin-producing E. coli.

Published by Elsevier Inc.